SYNTHESIS OF FLUORESCENT PROBES FOR CELL STUDIES

用于细胞研究的荧光探针的合成

• 批准号: 6281156
• 负责人: ALAN S VERKMAN
• 金额: $ 0.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281156
• 关键词: biomedical resource; minerals; respiratory system; spectrometry; structural biology; biomedical resource; minerals; respiratory system; spectrometry; structural biology

We have developed novel optical techniques to study solute dynamics in living cells and the kinetics of vesicular trafficking. Our laboratory has a full-time organic chemist who synthesizes specific fluorescent indicators for measurement of pH, chloride and solute dynamics in cytoplasm and certain subcellular compartments including and nucleus and endosomal secretary compartments. We utilize the mass spectrometry facility for confirmation of the structure of synthetic fluorescent probes.

我们已经开发了新颖的光学技术来研究溶质 活细胞的动力学和囊泡贩运动力学。 我们的实验室有一位全职有机化学家，他合成 特定的荧光指标，用于测量pH，氯化物和 细胞质和某些亚细胞隔室中的溶质动力学 包括和核和内体秘书室。 我们 利用质谱设施来确认 合成荧光探针的结构。