CORE--MAGNETIC RESONANCE IMAGING FACILITY

核心——磁共振成像设备

• 批准号: 2757396
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2757396
• 关键词: biomedical facility; embryo /fetus tissue transplantation; magnetic resonance imaging; nervous system transplantation; spinal cord injury

The primary mission of the magnetic resonance imaging (MRI) Core is to provide a detailed view of in vivo anatomy that is essential for the fulfillment of the objectives of the three Subprojects of this Program Project. Each MRI time point has been carefully selected to obtain specific information that will help to a) determine the severity of spinal cord injury, b) guide the transplant or vehicle injections, and c) determine transplant viability at key points (e.g., immediately prior to Cyclosporine A withdrawal). In addition, we will investigate the potential of MR microscopy for providing detailed 3-dimensional images of the injury or transplant site in selected postmortem spinal cord specimens. Since these data can be acquired in a time-efficient and cost-effective manner, we will assess the accuracy and reliability of MR microscopy for measuring important morphological parameters (e.g., lesion volume). Accordingly, the specific aims of this core are: (i) to provide in vivo anatomical views of the spinal cord at key time points during the injury and transplantation protocols, and (ii) to determine the ability of MR microscopy to provide reliable measures of several gross morphological parameters relevant to spinal cord injury and fetal tissue grafting. The MR images in Aim 1 will (a) provide an early evaluation of lesion severity by demonstrating hemorrhage and edema, (b) show lesion volume when locomotor function has stabilized, (c) show the lesion location and volume 1 week prior to delayed transplantation, which will assist greatly in determining the volume of the injection material, and (d) determine whether a viable graft is present 1 week prior to withdrawal of Cyclosporine A. Aim 2 will determine how accurately MR microscopy can illustrate lesion volume, transplant volume, and host- graft apposition, as well as white matter sparing in the adjacent host spinal cord. In addition, the noninvasive nature of the in vivo MRI studies underscores the clinical relevance of this Program Project, and the data obtained herein will be invaluable to any future cellular grafting approaches aimed at repair of human spinal cord injury.

磁共振成像（MRI）核心的主要任务是 提供体内解剖结构的详细视图，这对于 实现该程序的三个子项目的目标 项目。 每个MRI时间点已仔细选择以获得 有助于a）确定严重性的特定信息 脊髓损伤，b）指导移植或车辆注射，然后 c）确定关键点处的移植生存能力（例如，紧接之前 戒断环孢菌素）。 此外，我们将调查 MR显微镜提供详细的3维图像的潜力 选定的验尸脊髓中的损伤或移植部位 标本。 由于这些数据可以以时间效率和 具有成本效益的方式，我们将评估MR的准确性和可靠性 测量重要形态参数的显微镜（例如病变 体积）。 因此，该核心的具体目的是：（i）提供 在关键时间点脊髓的体内解剖学观点 伤害和移植方案，以及（ii）确定能力 MR显微镜的可靠度量 与脊髓损伤和胎儿组织有关的形态学参数 嫁接。 AIM 1中的MR图像将（a）提供早期评估 通过证明出血和水肿的病变严重程度，（b）显示病变 体积当运动功能稳定时，（c）显示病变 位置和卷延迟移植前1周，这将 极大地帮助确定注射材料的数量，并 （d）确定是否存在可行的移植物。 撤回环孢菌素A. AIM 2将确定MR的准确程度 显微镜可以说明病变体积，移植体积和宿主 - 移植物的申请以及相邻主机中保留的白质 脊髓。 此外，体内MRI的无创性 研究强调了该计划项目的临床相关性， 本文获得的数据对于将来的任何蜂窝都无价 旨在修复人脊髓损伤的嫁接方法。