BIOLOGY OF HUMAN AIDS RETROVIRUS

人类艾滋病逆转录病毒的生物学

• 批准号: 6098941
• 负责人: Bruce Chesebro
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6098941
• 关键词: HIV envelope protein; human immunodeficiency virus 1; human tissue; macrophage; microorganism culture; nucleic acid sequence; protein sequence; vascular endothelium; virus genetics; virus infection mechanism; virus replication

The primary goal of this project is to determine the role of variation in HIV sequences in causing the various clinical manifestations seen in AIDS. In vivo env sequence analysis of viruses associated with dementia suggested that unique viral strains may be responsible for the occurrence of HIV dementia. In contrast, other analyses indicated that dementia was not associated with higher levels of viral DNA. The influence of the V3 region of the envelope gene on macrophage tropism and the NSI and SI phenotypes was mapped to individual V3 amino acids, and it was found that usually macrophage tropism and the SI phenotype were independently determined by amino acids at different V3 positions. This finding provides an explanation for the occurrence of HIV strains which exhibit both the SI phenotype and macrophage tropism. More recently infectious HIV molecular clones with mutations in and near the V3 region were studied for their utilization of specific coreceptors and for their behavior in SCID /HuPBL mice

该项目的主要目标是确定 HIV序列变异在引起各种临床症状中的作用 艾滋病中常见的表现。体内 env 序列分析 与痴呆症相关的病毒表明，独特的病毒株 可能是艾滋病痴呆症发生的原因。在 相比之下，其他分析表明痴呆症与 具有较高水平的病毒DNA。 V3区的影响 巨噬细胞趋向性的包膜基因以及 NSI 和 SI 表型被映射到单个 V3 氨基酸，并且它是 发现通常巨噬细胞趋向性和 SI 表型 由不同V3位置的氨基酸独立决定。 这一发现为艾滋病毒的发生提供了解释 同时表现出 SI 表型和巨噬细胞的菌株 向性。最近感染性 HIV 分子克隆 研究了 V3 区及其附近的突变 SCID 中特定辅助受体的利用及其行为 /HuPBL 小鼠