CONTACT LENS EFFECTS ON HUMAN OCULAR MUCIN

隐形眼镜对人眼粘蛋白的影响

• 批准号: 2467527
• 负责人: Nancy A McNamara
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2467527
• 关键词: Pseudomonas aeruginosa; RNA; clinical research; contact lens; corneal epithelium; densitometry; density gradient ultracentrifugation; disease /disorder proneness /risk; enzyme activity; eye infections; gel electrophoresis; gel filtration chromatography; gene expression; glycoproteins; host organism interaction; human genetic material tag; human subject; keratitis; medical rehabilitation related tag; mucins; polymerase chain reaction; protein binding; southern blotting; tear

The goal of this project is to continue my development as an independent scientist and teacher. To achieve this goal, a five-year postdoctoral training program is proposed that will supplement my experience in clinical corneal research, with advanced training in the basic science disciplines of ocular surface biochemistry, molecular biology, microbiology, and bacterial pathogenesis. The program will include courses, seminars and laboratory experience at the Universities of California, San Francisco (UCSF) and Berkeley (UCB). Both UCSF and UCB document incredibly strong research programs in molecular and cell biology, biochemistry, and chemistry, and several key faculty will serve as sponsors. The proposed research project has been carefully designed to complement and enhance those skills obtained during the development period and will provide me with the necessary expertise to analyze the pathogenesis of various ocular surface diseases. Ocular mucins have been shown to protect against corneal infection by inhibiting Pseudomonas aeruginosa adherence to the ocular surface. The purpose of this project is to test the hypothesis that contact lens wear alters the quantity or composition of ocular mucins and thereby interferes with bacterial binding to tear film glycoproteins. The initial phase of this project will investigate the effects of hydrogel contact lens wear on the quantity and composition of secreted ocular mucins. Mucin samples will be obtained by irrigating the human ocular surface, and the optimal technique for purifying mucous glycoproteins will be determined. The quantity of secreted mucin and mucin RNA levels will be compared in contact lens wearers versus controls, and the composition of ocular mucin will be assessed using lectins and specific exoglycosidases. The second phase of experiments will examine the nature of the interaction between P. aeruginosa and ocular mucins, using competitive inhibition studies, and will determine whether or not contact lens wear affects this interaction. The risk of sight-threatening corneal ulceration resulting from overuse of hydrogel lenses is a significant public health concern, and this project will analyze whether or not changes to ocular mucin contribute to this risk. Understanding the effects of contact lens wear on ocular mucin may aid in the design of strategies to prevent or lessen the risk of ulcerative keratitis. This program and research experience will integrate my clinical and basic science training and enable me to formulate hypotheses about clinically observed phenomenon based upon a firm understanding of fundamental ocular biology.

该项目的目标是继续我作为独立的发展 科学家和老师。 为了实现这一目标，五年的博士后 提出了培训计划，以补充我的经验 临床角膜研究，接受基础科学的高级培训 眼科生物化学的学科，分子生物学， 微生物学和细菌发病机理。 该程序将包括 大学的课程，研讨会和实验室经验 加利福尼亚，旧金山（UCSF）和伯克利（UCB）。 UCSF和UCB 记录了分子和细胞中令人难以置信的强大研究计划 生物学，生物化学和化学，几个关键教师将服务 作为赞助商。 拟议的研究项目经过精心设计 补充和增强开发过程中获得的这些技能 时期，将为我提供必要的专业知识来分析 各种眼表疾病的发病机理。 眼粘蛋白具有 被证明可以通过抑制来预防角膜感染 铜绿假单胞菌依从于眼表面。 目的 该项目是为了测试隐形眼镜穿着改变的假设 眼粘蛋白的数量或组成，从而干扰 细菌与泪膜糖蛋白的结合。最初的阶段 项目将研究水凝胶隐形眼镜磨损的影响 分泌的眼粘蛋白的数量和组成。 粘蛋白样品 将通过灌溉人眼表面获得，最佳 将确定纯化粘液糖蛋白的技术。 这 将比较数量的分泌粘蛋白和粘蛋白RNA水平 隐形眼镜佩戴者与对照组的组成 粘蛋白将使用凝集素和特定外糖苷酶进行评估。 这 实验的第二阶段将检查相互作用的性质 使用竞争性抑制，在铜绿假单胞菌和眼粘蛋白之间 研究，并将确定隐形眼镜磨损是否影响 这种相互作用。 视力危及角膜溃疡的风险 过度使用水凝胶镜头是重要的公共卫生 关注的问题，该项目将分析对眼的变化 粘蛋白有助于这种风险。 了解接触的影响 眼粘蛋白的镜头磨损可能有助于设计策略以防止 或减少溃疡性角膜炎的风险。该计划和研究 经验将整合我的临床和基础科学培训，以及 使我能够对临床观察到的现象提出假设 基于对基本眼生物学的坚定理解。