CORE--CFAR FLOW CYTOMETRY/CELL SORTING

核心--CFAR流式细胞术/细胞分选

• 批准号: 6324588
• 负责人: JANIS V GIORGI
• 金额: $ 14.28万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6324588
• 关键词: AIDS; HIV infections; biohazard control; biomedical facility; cell sorting; experimental designs; flow cytometry; human immunodeficiency virus; training

The purpose of the CFAR Flow Cytometry/Cell Sorting Core (Core D), referred to here as the "CFAR Flow Core", is to provide specialized flow cytometry to all CFAR investigators, to foster multi-disciplinary interactions within the UCLA research community, and to promoter advances in AIDS research. The specific aims of the Core are to: (1) provide flow cytometry instrumentation and technical assistance for experiments; (2) consult on flow cytometry; (3) provide training in the use of flow cytometry instrumentation and procedures; (4) maintain the quality of all data produced from the laboratory; (5) facilitate convenient, economical data handling for the users; and (6) provide support services to users. The CFAR Flow Core, consisting of 1.7 FTEs and an annual budget of $128,500, operates within the framework of the overall UCLA Research Flow Cytometry Core Facility which maintain 5.1 FTEs and has an overall budget of $389,577. Equipment available in the laboratory to CFAR investigators includes two FACScans and two FACStar/plus cell sorters. Of particular importance to the UCLA CFAR is that both of the FACStar/plus cell sorters are housed and maintained such that they can be used for cell separation of potentially biohazardous specimens, thereby permitting sorting of cells from HIV-infected people and sorting of cultured cells infected in vitro with HIV. A safety protocol, implemented to ensure the protection of the operator and the researchers during cell sorting of biohazardous samples, including isolated rooms, barrier protection of the operator, meticulous machine maintenance, and periodic tests to verify aerosol containment. The efficiency with which the Core can offer sorting of HIV-infected specimens as an invaluable asset of the UCLA CFAR. Center investigators also have ready access to routine as well as sophisticated analytic flow cytometric techniques. Among the sophisticated techniques available that have been user by CFAR investigators are four-color immunofluorescence analysis, CA++ flux measurements and assessments of apoptosis. The supervisor, who has 14 years of flow cytometry experiences, assists investigators with advice on experimental design, assistance with obtaining specialized reagents, consultation on data interpretation, training of new users, and development of new flow cytometric techniques or modification of existing techniques to meet emerging research needs. The CFAR Core gives priority in assignment of time to projects that foster translational research and those that involve basic and clinical research collaborations.

CFAR流式细胞仪/细胞排序核心（核心D）的目的， 这里称为“ CFAR流核”，是为了提供专业流 对所有CFAR研究人员的细胞仪，以培养多学科 UCLA研究社区内的互动，以及促进者的进步 在艾滋病研究中。核心的具体目的是：（1）提供流程 用于实验的细胞仪仪器和技术援助； （2） 咨询流式细胞仪； （3）提供流量使用的培训 细胞仪仪器和程序； （4）保持所有人的质量 从实验室产生的数据； （5）方便，经济的 用户的数据处理； （6）为用户提供支持服务。 CFAR流核，由1.7 FTE和年度预算组成 $ 128,500，在整体UCLA研究流的框架内运营 维持5.1 FTE的细胞仪核心设施，并具有总体预算 389,577美元。 CFAR调查人员在实验室中可用的设备 包括两个FACSCAN和两个FACSTAR/PLUS细胞分选器。特别 对UCLA CFAR的重要性是两个Facstar/Plus Cell Sorters 被安装和维护，使它们可用于细胞分离 潜在的生化标本，从而允许分类细胞 来自感染HIV的人和体外感染的培养细胞 与艾滋病毒。安全协议，以确保保护 运营商和研究人员在生物危害样品的细胞分类过程中， 包括孤立的房间，操作员的障碍保护，细致 机器维护和定期测试以验证气溶胶遏制。这 核心可以提供的效率可以提供艾滋病毒感染标本的排序 作为UCLA CFAR的宝贵资产。中心调查人员也有 准备访问常规以及复杂的分析流式细胞仪 技术。在可用的复杂技术中 CFAR研究人员的用户是四色免疫荧光分析， CA ++通量测量和凋亡的评估。主管，谁 有14年的流式细胞仪经验，协助研究人员 有关实验设计的建议，协助获得专业 试剂，数据解释咨询，新用户培训以及 开发新的流式细胞仪技术或现有的修改 满足新兴研究需求的技术。 CFAR核心优先 分配时间到促进翻译研究和的项目 那些涉及基本和临床研究合作的人。