PRENATAL ETHANOL EXPOSURE AND POSTNATAL HANDLING

产前乙醇暴露和产后处理

• 批准号: 6224419
• 负责人: KARA Irene GABRIEL
• 金额: $ 0.63万
• 依托单位: UNIVERSITY OF BRITISH COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6224419
• 关键词: behavioral /social science research tag; behavioral habituation /sensitization; embryo /fetus toxicology; environmental adaptation; ethanol; ethology; fetal alcohol syndrome; hormone regulation /control mechanism; hypothalamic pituitary adrenal axis; laboratory rat; learning; newborn animals; performance; psychological tests; stimulus /response; stress; touch

The proposed research is designed to investigate the effects of early postnatal handling as an environmental manipulation which may attenuate the adverse behavioral and physiological consequences of prenatal ethanol exposure. This research will examine the possible mechanisms mediating the hormonal hyperresponsiveness to stressors observed in animals prenatally exposed to ethanol (E) as well as the possible influence of postnatal handling on these mechanisms. In addition, experiments will investigate the correspondence between prenatal ethanol-induced alterations in behavior and HPA activity. Toward achievement of these aims, experiments will involve behavioral and hormonal assessments. Tasks involving behavioral inhibition (conditioned taste aversion) and spatial learning (Morris water maze) will explore further the learning and/or performance deficits previously demonstrated in E animals as well as investigate the correspondence between behavior and HPA activity. Functional manipulations of the hypothalamic-pituitary-adrenal (HPA) axis will examine possible mechanisms of the altered hormonal responsiveness to stress observed in E animals as well as explore the effects of handling on this HPA hyperresponsiveness. Lastly, experiments will investigate the effects of prenatal ethanol exposure and postnatal handling on activity of the HPA axis under basal conditions to determine if altered steady state activity contributed to HPA differences following stress.

拟议的研究旨在调查早期的影响 产后处理作为一种环境操纵，可能会减弱 产前不良行为和生理后果 乙醇暴露。 这项研究将探讨可能的机制 调节荷尔蒙对应激源的过度反应 产前接触乙醇 (E) 的动物以及可能的 产后处理对这些机制的影响。 此外， 实验将调查产前之间的对应关系 乙醇诱导的行为和 HPA 活性改变。 为了实现这些目标，实验将涉及行为 和荷尔蒙评估。 涉及行为抑制的任务 （条件性味觉厌恶）和空间学习（莫里斯水迷宫） 将进一步探讨之前的学习和/或表现缺陷 在 E 动物中证明并调查对应关系 行为和 HPA 活动之间的关系。 的功能操纵 下丘脑-垂体-肾上腺 (HPA) 轴将检查可能的情况 观察到的荷尔蒙对压力反应改变的机制 E 动物以及探索处理对这种 HPA 的影响 过度反应。 最后，实验将研究效果 产前乙醇暴露和产后处理对乙醇活性的影响 基础条件下的 HPA 轴以确定稳态是否改变 活动导致压力后 HPA 差异。