CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6100441
• 负责人: MON-LI H. CHU
• 金额: $ 14.48万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6100441
• 关键词: biomedical facility; computer assisted sequence analysis; epidermolysis bullosa; evaluation /testing; gel electrophoresis; genetic library; genetic techniques; nucleic acid biosynthesis; nucleic acid sequence; nucleotides; polymerase chain reaction; reagent /indicator; tissue /cell culture

The Tssue Culture Core facilities are of central importance for this Program Project, as they will continue to provide cultured cells from individuals affected with EB, from their unaffected family members, and from unrelated healthy controls for study in all Projects. During the previous years of support, the tissue culture facility has handled a large number of human cells from EB and control subjects. It has also maintained a large number of either spontaneously or virally transormed human cell lines that have been extremely helpful in elucidating gene expression of BMZ macromolecules, including type VII collagen. These established cell lines include a human amniotic endothelial cell line (WISH), a human epidermoid oral carcinoma cell line (KB), and a human fibrosarcoma cell line (HT-1080), which are commercially available (ATCC).

津江文化核心设施对此至关重要 计划项目，因为他们将继续提供来自 患有 EB 的个人及其未受影响的家庭成员，以及 来自所有项目研究中不相关的健康对照。 期间 在前几年的支持下，组织培养设施已经处理了 来自 EB 和对照受试者的大量人类细胞。 它还具有 维持大量自发或病毒转化 对阐明基因非常有帮助的人类细胞系 BMZ 大分子的表达，包括 VII 型胶原蛋白。 这些 已建立的细胞系包括人羊膜内皮细胞系 (WISH)、人表皮样口腔癌细胞系 (KB) 和人 纤维肉瘤细胞系（HT-1080），可商购 （ATCC）。