MOLECULAR AND GENETIC ANALYSIS OF MUSCLE SPECIFIC GENES

肌肉特异性基因的分子和遗传分析

• 批准号: 6240493
• 负责人: Greg L Harris
• 金额: $ 3.66万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240493
• 关键词: Drosophilidae; cytogenetics; developmental genetics; gene expression; in situ hybridization; molecular cloning; muscle cells; muscle contraction; muscle function; nucleic acid probes; transposon /insertion element

Muscle cells possess a number of unique functional characteristics which set them apart from other cell types in higher organisms - most notably those related to excitability, the coupling of an electrical signal into muscle contraction and the contractile process itself. The long term goals of the proposed research are to understand these cellular processes at the level of the molecular mechanisms which underlie muscle cell function. The immediate aims of the proposed work will be to begin to isolate and to characterize genes which we have putatively identified to be expressed specifically in muscle cells of adult organisms and not in other cell types. It is postulated that these genes may encode proteins that are involved in the unique processes of the muscle cell. This work will entail the use of a recently devised molecular scheme involving the fruitfly Drosophila, which allows one to identify easily the spatial and temporal expression pattern of genes which have been mutagenized or are close to the site of a mutation generated by the insertion of genetically engineered transposable p-elements into the genome. Once identified, the muscle-specific genes will be subjected to further molecular analyses which include cytogenetic mapping of the physical location of the genes on polytene chromosomes and the isolation, cloning and characterization of genes of interest. Ultimately this approach may aid in understanding the structure, function and role of proteins involved in muscle cell function. Funds are requested to support one undergraduate and one graduate student. The undergraduate student will learn basic laboratory skills and specific skills related to his/her project under the direction of the P.I. and will be supervised on a daily basis by a postdoctoral fellow or advanced graduate students. By the second year, the undergraduate will be encouraged to work independently on a portion of the proposed studies. The graduate student will be supervised by the P.I. and will learn to become an independent scientist. All students will attend weekly lab meetings and seminars and will be expected to communicate the results of their studies at regional and appropriate national meetings.

肌肉细胞具有许多独特的功能特征， 将它们与高等生物中的其他细胞类型区分开来 - 最值得注意的是 与兴奋性有关的那些，将电信号耦合到 肌肉收缩和收缩过程本身。 长期来看 拟议研究的目标是了解这些细胞过程 在肌肉细胞的分子机制水平上 功能。 拟议工作的近期目标将是开始 分离并表征我们推定已识别的基因 特异地在成体生物体的肌肉细胞中表达，而不是在 其他细胞类型。 推测这些基因可能编码蛋白质 参与肌肉细胞的独特过程。 这部作品 将需要使用最近设计的分子方案，涉及 果蝇，它可以让人们轻松识别空间和 已被诱变或被诱变的基因的时间表达模式 靠近因插入基因而产生突变的位点 将转座p元件工程化到基因组中。 一旦确定， 肌肉特异性基因将接受进一步的分子分析 其中包括基因物理位置的细胞遗传学图谱 多线染色体及其分离、克隆和表征 感兴趣的基因。 最终这种方法可能有助于理解 肌肉细胞中蛋白质的结构、功能和作用 功能。 请求资金支持一名本科生和一名研究生 学生。 本科生将学习基本的实验室技能 以及在他/她的指导下与他/她的项目相关的具体技能 P.I.并将由博士后研究员或博士后进行日常监督 高级研究生。 到第二年，本科生将 鼓励独立开展部分拟议研究。 研究生将受到 P.I. 的监督。并将学会 成为一名独立科学家。 所有学生将参加每周实验室 会议和研讨会，并预计传达会议和研讨会的结果 他们在区域和适当的国家会议上进行的研究。