CORE--CYTOKINE ANALYSIS

核心--细胞因子分析

• 批准号: 6296251
• 负责人: KENNETH W BEAGLEY
• 金额: $ 4.21万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6296251
• 关键词: DNA footprinting; RNase protection assay; bioassay; biomedical facility; cytokine; enzyme linked immunosorbent assay; gene expression; in situ hybridization; messenger RNA; northern blottings; polymerase chain reaction; southern blotting

The Cytokine Core will assist RCOB investigators with cytokine analysis. Analyses will include cytokine determination of media and fluids by bioassays and ELISA, and cytokine mRNA expression by Dot/Northern analysis, S1 analysis, RNAse protection analysis, RT-PCR and in situ hybridization. the core will also provide advice and expertise in the planning of cytokine-related experiments. the core at this time has available cDNA probes for human (IL-1 through IL-10, IFN-gamma, TGF- alpha, TNF-alpha/beta) and for murine (IL-1 through IL-10, TNF- alpha/beta, TGF-alpha, IFN-gamma) cytokines. Bioassays presently available include IL-1 (human and mouse). In addition, ELISA's for murine IL-5 and IFN-gamma are also available. Neutralizing antibodies used in these bioassays will also be available to researchers at UAB through the Core Facility. The core is fully equipped with instrumentation for these analyses and has developed the necessary expertise to conduct the proposed studies. The Core will be used extensively by Projects 8 and 5 and occasionally by Projects 4 and 3.

细胞因子核心将帮助RCOB研究者进行细胞因子分析。 分析将包括细胞因子确定培养基和流体的测定 生物测定和ELISA，以及DOT/Northern的细胞因子mRNA表达 分析，S1分析，RNase保护分析，RT-PCR和原位 杂交。 核心还将在 与细胞因子相关实验的计划。 此时的核心有 可用人类的cDNA探针（IL-1至IL-10，IFN-GAMMA，TGF- alpha，tnf-alpha/beta）和鼠（IL-1至IL-10，TNF- alpha/beta，TGF-Alpha，IFN-Gamma）细胞因子。 生物测定现在 可用包括IL-1（人和小鼠）。 此外，Elisa的 也可以使用Murine IL-5和IFN-Gamma。 中和抗体 在这些生物测定中也将用于UAB的研究人员 通过核心设施。 核心完全配备了 这些分析的仪器，并开发了必要的 进行拟议研究的专业知识。 核心将被使用 由项目8和5广泛，偶尔由项目4和3进行。