RESIN/DENTIN INTERDIFFUSION ZONE

树脂/牙本质相互扩散区

• 批准号: 6238496
• 负责人: SALLY J MARSHALL
• 金额: $ 26.66万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6238496
• 关键词: adhesions; atomic force microscopy; biomaterial interface interaction; collagen; composite resins; dental bonding material; dental stress analysis; dental structure; dentin; human tissue; physical property; scanning electron microscopy; transmission electron microscopy

An important basic concept in this proposal is that dentin structural characteristics and properties are the key considerations for future development of dentin bonding systems. The most promising approach thus far is the formation of the resin-dentin interdiffusion zone (also known as resin hybrid layer or resin reinforced layer). The fundamental concept of the resin hybrid layer is that it provides bonding to dentin through establishment of intimate mechanical entanglement between partially demineralized dentin and dentin bonding agents that penetrate this structure and are polymerized in situ. The resin component of this newly formed composite structure can then be chemically bonded to other polymeric agents and composites. This concept has provided significant improvements in dentin bonding. Many key questions remain to be answered concerning dentin associated variables which impact the formation of the interdiffusion zone. In this project the impact of the following variables on structure and properties of this zone will be addressed: 1) nature of adhesive system; 2) dentin depth; 3) alterations of collagen fibril structure at each bonding step; 4) altered and clinically important forms of dentin; and 5) moist vs dry bonding. The substructure and properties of the resin-dentin interdiffusion zone of a modern dentin bonding system and an advanced single component bonding system will be compared as a function of intratooth location in aim 1 using a novel reverse sandwich method allowing study of superficial and deep dentin in the same sample. Methods will include SEM, AFM nano-hardness, and TEM. The hypothesis that the resin-dentin interdiffusion zones prepared in transparent dentin of coronal caries and sclerotic dentin of cervical lesions exhibit altered substructure and properties as compared to normal dentin will be tested (aim 2) using similar methods. Alterations in the collagen fibril network at each of the steps associated with formation of the resin-dentin interdiffusion zone will be determined using high resolution AFM (aim 3). The structure and properties of the interdiffusion zones formed when bonding to wet and dry dentin will be determined using AFM nano-hardness, TEN and SEM methods (aim 4). Micro- punch shear strength (aim 5) will be used as a method to detect differences in the strength of altered forms of dentin and interdiffusion zones. These comparisons will evaluate the different adhesive systems, using moist and dry bonding methods, and the role of superficial normal dentin, deep normal dentin, the transparent caries layer and sclerotic cervical dentin.

该提案中的一个重要基本概念是牙本质结构 特征和属性是未来的关键考虑因素 牙本结合系统的开发。 因此，最有前途的方法 远方是树脂丁丁互嵌带区的形成（也已知 作为树脂混合层或树脂增强层）。 基本概念 树脂混合层的是，它通过 建立部分机械纠缠 脱矿的牙本质和牙本质粘合剂穿透了这一点 结构并原位聚合。 这个新的树脂成分 然后可以将形成的复合结构化学键合到其他 聚合物剂和复合材料。 这个概念提供了重要的 牙本结合的改善。 许多关键问题仍有待回答 关于影响牙本质相关的变量，这些变量会影响形成 扩散区。 在这个项目中，以下影响 将解决有关该区域结构和属性的变量：1） 粘合系统的性质； 2）牙本质深度； 3）胶原蛋白的改变 每个粘结步骤处的原纤维结构； 4）改变和临床重要 牙本质的形式； 5）潮湿与干键合。 子结构和 现代牙本质的树脂丁丁互化区的特性 粘合系统和高级单个组件键合系统将是 使用新颖 反向三明治方法允许研究浅表和深牙本质 相同的样本。 方法将包括SEM，AFM纳米硬度和TEM。 树脂丁丁交叉扩散区的假设 冠状龋齿的透明牙本质和颈椎硬化牙本质 与正常人相比，病变表现出改变的子结构和特性 将使用类似方法对牙本质进行测试（AIM 2）。 改变 与形成相关的每个步骤 树脂丁丁互扩散区将使用高 解决方案AFM（目标3）。 的结构和特性 与潮湿和干牙本结合时形成的扩散区将是 使用AFM纳米硬度，十和SEM方法（AIM 4）确定。 微- 打孔剪切强度（AIM 5）将用作检测的方法 牙本质和扩散形式改变形式的强度差异 区域。 这些比较将评估不同的粘合剂系统， 使用湿润和干燥的键合方法，以及表面正常的作用 牙本质，深法向牙本质，透明龋齿层和硬化 宫颈牙本质。