Clash of the Kingdoms: How the quest for nutrients leads to pathogenicity

王国的纷争：对营养的追求如何导致致病性

• 批准号: NE/M001415/1
• 负责人: Paul Hoskisson
• 金额: $ 55.23万
• 依托单位: University of Strathclyde
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FM001415%2F1
• 关键词: Clash; Kingdoms; How; quest; nutrients

Streptomyces is a harmless soil bacterium that is often found around the roots of plants, interacting with them but without causing disease. Streptomyces also interacts with other soil organisms such as amoeba, single celled organisms that behave like the phagocytic cells in the human body, eating bacterial cells. Currently we know very little about how these different species interact at the molecular level. Recently we were able to identify that a group of genes (the mce genes, which stands for Mammalian Cell Entry genes)that are found in Streptomyces and are very closely related to genes in its distant cousin Mycobacterium tuberculosis, the bacterium that causes TB. We showed that these genes encode a transport system and are important for Streptomyces to gather nutrients from plant roots and also to interact with the amoebae. Yet in M. tuberculosis they are required for them to colonise human lung cells and grow within those cells. In this proposal we will fully characterise this transport system and study the molecular details of what the proteins encoded by these genes do, what substrates they bind and take up, and what effect they have on plant and amoeba both physically and also genetically. The findings of this study will have implications for our understanding of how human and animal pathogens have evolved from non-pathogenic bacteria and how bacteria can colonise plants and out-compete plant pathogens to stop them from causing disease. There are also possibilities that these genes can be used in the biotechnology industry to help make useful drugs, such as steroids from plant waste products.

链霉菌是一种无害的土壤细菌，经常在植物的根部周围发现，与它们相互作用，但不会引起疾病。链霉菌还与其他土壤生物（例如变形虫，单细胞生物）相互作用，其表现像人体中的吞噬细胞一样，食用细菌细胞。目前，我们对这些不同物种在分子水平上的相互作用方式了解甚少。最近，我们能够确定在链霉菌中发现的一组基因（代表哺乳动物细胞进入基因的MCE基因）与其遥远的Cousin结核病结核病中的基因密切相关，该基因是导致TB的细菌。我们表明这些基因编码了一个传输系统，对于链霉菌以从植物根中收集营养并与变形虫相互作用很重要。然而，在结核分枝杆菌中，他们需要将人类肺细胞定居并在这些细胞内生长。在此提案中，我们将充分表征该运输系统，并研究这些基因编码的蛋白质所做的蛋白质的分子细节，它们结合和采用的底物以及它们对植物和植物的影响既有物理和遗传性。这项研究的发现将对我们了解人类和动物病原体如何从非致病细菌进化出来，以及细菌如何在植物上定位并超出植物病原体以阻止其引起疾病。也有可能在生物技术行业中使用这些基因来帮助制造有用的药物，例如植物废物的类固醇。

项目成果

Merging In-Solution X-ray and Neutron Scattering Data Allows Fine Structural Analysis of Membrane-Protein Detergent Complexes.

合并溶液内 X 射线和中子散射数据可以对膜-蛋白质洗涤剂复合物进行精细结构分析。

• DOI: 10.1021/acs.jpclett.8b01598
• 发表时间: 2018
• 期刊: The journal of physical chemistry letters
• 作者: Dias Mirandela G

Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences.

• DOI: 10.1038/s41598-018-35005-2
• 发表时间: 2018-11-12
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Albanna A;Sim M;Hoskisson PA;Gillespie C;Rao CV;Aldridge PD
• 通讯作者: Aldridge PD

Single-shot time-gated fluorescence lifetime imaging using three-frame images.

• DOI: 10.1364/oe.26.017936
• 发表时间: 2018-06
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Yahui Li;Hui Jia;Shaorong Chen;Jinshou Tian;Lingliang Liang;Fenfang Yuan;Hongqi Yu;D. Li