A NEW IN VIVO HUMAN VAGINAL XENOGRAFT MODEL

一种新的体内人类阴道异种移植模型

• 批准号: 6235308
• 负责人: JOHN W KREIDER
• 金额: $ 11.86万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235308
• 关键词: SCID mouse; acidity /alkalinity; antiinfective agents; athymic mouse; bacteria; cytotoxicity; disease /disorder model; drug screening /evaluation; estradiol; estrogen receptors; human tissue; model design /development; oral contraceptives; progesterone; progesterone receptors; sperm; spermicide; vagina; xanthine oxidase; xenotransplantation

Model systems for microbicide evaluations are inadequate. In vitro tests can provide support for drug efficacy but cannot reproduce the complex vaginal environment. The vaginas of experimental animals are poorly characterized and do not provide a sufficient representation of the human counterpart. In this project, we will modify our human xenograft system so that grafts of human vagina can be used for microbicide evaluation. The Specific Aims of the study are as follows: 1. Define the Composition of human Vaginal Xenografts 2. Determine Human Vaginal Xenografts Responsiveness to Sex Steroids 3. Test the Susceptibility of Human Vaginal Xenografts to Primary infections with Selected Pathogens 4. Determine the Effectiveness and Toxicity of Selected Microbicides on Pathogens in Human Vaginal Xenografts.

杀微生物剂评估的模型系统不充分。体外测试 可以为药物功效提供支持，但不能重现复合物 阴道环境。实验动物的阴道状况很差 特征，并且不能充分代表人类 对方。在这个项目中，我们将修改我们的人类异种移植系统，以便 人类阴道移植物可用于杀菌剂评价。这 研究的具体目的如下： 1. 定义人类阴道异种移植物的组成 2. 确定人类阴道异种移植物对性类固醇的反应 3. 测试人阴道异种移植物对原发性的敏感性 特定病原体感染 4. 确定所选微生物杀灭剂的有效性和毒性 人类阴道异种移植物中的病原体。