CHAR OF LOW BARRIER HYDROGEN BONDS IN SERINE PROTEASE & MODEL COMPOUNDS

丝氨酸蛋白酶中低势垒氢键的特征

• 批准号: 6298136
• 负责人: PERRY A. FREY
• 金额: $ 0.75万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6298136
• 关键词: biological products; biomedical resource; enzymes; nuclear magnetic resonance spectroscopy; proteins

This investigation deals with the importance of low-barrier hydrogen bonds in the structure and function of enzymes. We need to synthesize a series of trifluormethyl ketone inhibitors and then characterize low-barrier hydrogen bonds between inhibitors and enzyme by NMR spectroscopic techniques. We also need to determine isotope effects and fractionation factors of these compounds.

这项调查涉及低障碍的重要性 酶的结构和功能中的氢键。 我们需要 合成一系列三氟甲基酮酮抑制剂，然后 表征抑制剂和酶之间的低垒氢键 通过NMR光谱技术。 我们还需要确定同位素 这些化合物的作用和分级因子。