METAL-METAL INTERACTIONS IN THE KIDNEY

肾脏中的金属-金属相互作用

• 批准号: 6217648
• 负责人: A J GANDOLFI
• 金额: $ 12.65万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6217648
• 关键词: arsenic; atomic absorption spectrometry; autoradiography; biomarker; cadmium; chromium; cytotoxicity; emission spectrometry; environmental contamination; environmental toxicology; heavy metals; histochemistry /cytochemistry; histopathology; kidney cell; kidney function; laboratory rabbit; mercury; renal cortex; renal toxin; renal tubular transport; renal tubule; tissue /cell culture; tissue /cell preparation; toxicant interaction; waste disposal

Description: The investigators propose to examine the interactive accumulation, toxicity, and excretion of Hg, Cd, As, and Cr in rabbit kidney slices and in primary cultures of proximal renal tubular cells grown on porous membrane supports. The aim is to determine the effect of exposure to one metal on the kinetics and toxicity of the other metals, using in vitro systems and based on micro-PIXE analyses and biochemical indices of toxicity (e.g., K+/DNA ratio). The effects of certain metal ligands (GSH, MT, albumin) and chelators (DMPS) on the interactions will be tested.

描述：调查人员建议检查互动 Hg，Cd，AS和Cr的累积，毒性和排泄物在兔子中 肾切片和近端肾小管细胞的原发性培养物 在多孔膜支撑上生长。目的是确定效果 在动力学和另一种金属上接触一种金属 金属，使用体外系统，并基于微贴分析和 毒性的生化指标（例如K+/DNA比）。效果 某些金属配体（GSH，MT，白蛋白）和螯合剂（DMP） 相互作用将进行测试。