MACACA NEMESTRINA & HUMAN LYMPHOPOIETIC CHIMERAS

猕猴

• 批准号: 6116352
• 负责人: William Randall Morton
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116352
• 关键词: Mammalia; Primates; animal tissue; human tissue; model design /development; transplantation

In this project we hope to establish a stable xenogenic (macaque-human) lymphopoietic chimeric M. nemestrina. We hypothesize that these macaque-human chimerics will enhance the infectivity of HIV-1 in these species, and that the chimeric system will be capable of sustaining replication of the virus in vivo. This procedure is planned as follows fetal macaques, about 30-40 days gestation, will be inoculated with hematopoietic stem cells recovered from human umbilical cord blood or fetal liver tissue. One fetus inoculated with this procedure was born with a high level of engraftment and chimerism. Further work in this direction is warranted.

在这个项目中，我们希望建立一个稳定的异种（猕猴-人类）淋巴细胞嵌合M. nemestrina。 我们假设这些猕猴-人类嵌合体将增强这些物种中HIV-1的感染性，并且嵌合系统将能够维持病毒在体内的复制。 该程序计划如下：将在妊娠约30-40天的猕猴胎儿中接种从人脐带血或胎儿肝脏组织中回收的造血干细胞。 通过这种程序接种的一个胎儿出生时具有高水平的植入和嵌合状态。有必要朝这个方向进一步开展工作。