FUNCTIONAL ANALYSIS OF DROSOPHILA ERG POTASSIUN CHANNELS

果蝇 ERG 钾通道的功能分析

• 批准号: 6179155
• 负责人: BLAKE D ANSON
• 金额: $ 1.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6179155
• 关键词: Drosophilidae; Xenopus oocyte; animal genetic material tag; developmental neurobiology; gene induction /repression; gene mutation; invertebrate embryology; larva; molecular cloning; neurogenetics; neurons; potassium channel; protein structure function

The goal of this project is to understand the role of the Drosophila ether- -go-go (erg) K+ channel in membrane excitability. Specifically, the structure/function relationships of this protein, how this protein participates in membrane excitability, and the functional consequences of altering channel structure will be assessed through biophysical, neuronal, and mutational investigations. Since the human form (HERG) of this K+ channel is involved in potentially fatal cardiac arrhythmias, information gathered from this study should be directly relevant to the human condition. An examination of heterologously expressed erg K+ channels will define the biophysical parameters of this channel, allow comparisons to be made with other K+ channels, and provide information essential for study of this channel in its more native cellular environment. An examination of this channel in dissociated neurons will provide insight as to the role this channel plays in regulating membrane excitability as well as providing novel information on the neuronal function of this subfamily of K+ channels. A mutational analysis of erg K+ channels will allow elucidation of protein domains essential for channel function and demonstrate the consequences of altering such domains. Overall, this proposal will increase our understanding of K+ channel biology and provide insight as to the causes underlying disorders of membrane excitability.

该项目的目标是了解果蝇的作用 ether- -go-go (erg) 膜兴奋性中的 K+ 通道。 具体来说， 该蛋白质的结构/功能关系，该蛋白质如何 参与膜兴奋性及其功能后果 改变通道结构的能力将通过生物物理学进行评估， 神经元和突变研究。 自人类形态（HERG） 该 K+ 通道的一部分与潜在致命的心律失常有关， 从本研究中收集的信息应与研究直接相关 人类状况。 对异源表达的 erg K+ 通道的检查将定义 该通道的生物物理参数，可以进行比较 与其他 K+ 频道合作，并提供研究所需的信息 该通道处于更原生的细胞环境中。 一次考试 分离神经元中该通道的研究将提供关于 该通道在调节膜兴奋性以及 提供有关该亚科神经元功能的新信息 K+频道。 erg K+ 通道的突变分析将允许 阐明通道功能所必需的蛋白质结构域 展示改变这些域的后果。 总体而言，这 提案将增加我们对 K+ 通道生物学的理解 提供有关膜潜在疾病原因的见解 兴奋性。