STRUCTURE OF HYPERTROPHIC CENTROSOMES IN HUMAN BREAST TUMORS

人乳腺肿瘤肥大中心体的结构

• 批准号: 6121829
• 负责人: JEFFREY L SALISBURY
• 金额: $ 2.78万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121829
• 关键词: biomedical resource; clinical research; female; human subject; microscopy; neoplasm /cancer; reproductive system; women's health

We have shown through immunofluorescence and confocal microscopy that centrosomes of high grade breast tumors are larger and more numerous than centrosomes in normal breast tissue. Additionally, the centrosomes in breast tumor cells are hyperphosphorylated and are capable of nucleating a larger number of microtubules in vitro as compared to centrosomes from normal breast cells. We describe these centrosomes as "hypertrophic". Attempts to characterize hypertrophic centrosomes at an ultrastructural level using CTEM have been difficult. This is due, at least in part, to the small volume of the centrosomes relative to the total volume of the cells; the centrosomes are few and far between in 90 nm thick sections through cells that are 6-10 um thick. In addition, the hypertrophic centrosomes we have observed with CTEM have been especially electron opaque, making it difficult to resolve microtubules and other structural components of the centrosomes. Initial results using 0.5 and 1 micron thick sections have proven not only to greatly facilitate the locating of hypertropic centrosomes, but have also produced 3-dimensional images with heretofore unseen structural detail. Several tomographic reconstructions of centrosomes from cancerous cells were performed when Dr. Lingle visited the laboratory last summer. Additional reconstructions are planned in the near future.

我们已经通过免疫荧光和共聚焦显微镜展示了 高级乳腺肿瘤的中心体更大，更多 在正常乳腺组织中比中心体数量很多。 另外， 乳腺肿瘤细胞中的中心体被过度磷酸化，并且是 能够在体外构成大量的微管AS 与正常乳腺细胞的中心体相比。 我们描述了这些 中心体为“肥厚性”。 试图表征肥厚的 使用CTEM的超微结构级别的中心体一直是 难的。 这至少部分归因于少量 相对于细胞的总体积相对于中心体；中心体 在90 nm厚的部分中，很少有彼此之间的距离 6-10 UM厚。 另外，肥厚的中心体我们有 用CTEM观察到的是特别不透明的，使其成为 难以解决微管和其他结构组件的 中心体。 使用0.5和1微米厚的初始结果 事实证明，部分不仅可以极大地促进 肥大的中心体，但也产生了3维图像 迄今未见的结构细节。 几个断层扫描 从癌细胞中重建中心体的重建 去年夏天，Lingle博士访问了实验室。 额外的 计划在不久的将来进行重建。