CATION BINDING IN AQUEOUS BIOPOLYMER SYSTEMS

水性生物聚合物体系中的阳离子结合

基本信息

批准号：
6205898
负责人：
DONALD E WOESSNER
金额：
--
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-08-15 至 2000-08-14
项目状态：
已结题

项目摘要

We continue to apply 13C NMR methods for analysis of metabolism in the isolated rat heart. Over the last year one target has been analysis of metabolism in hypertrophied heart tissue. Earlier studies indicated that energy production from exogenous fatty acids or carbohydrates and from endogenous energy stores may be substantially disrupted in left ventricular hypertrophy. The contribution of long chain fatty acids (0.35 mM), lactate (1.2 mM), pyruvate (0.09 mM), glucose (5.5 mM), acetoacetate (0.12 mM), a-hydroxybutyrate (0.05 mM), and endogenous sources to energy production was determined by 13C NMR isotopomer analysis in two standard models of left ventricular hypertrophy, the spontaneously hypertensive rat and the aortic banded Sprague-Dawley rat. The fraction of acetyl-CoA derived from various sources was not different between the two control groups: fatty acids, 0.46-0.56; lactate plus pyruvate, 0.25-0.32; ketones, 0.11-0.12; glucose, 0.03-0.05; and endogenous sources, 0.03-0.06. However, oxidation of fatty acids was significantly decreased in spontaneously hypertensive rats (0.20 q 0.02 of acetyl-CoA production, mean q s.d.) or in banded Sprague-Dawley rats (0.24 q 0.04 of acetyl-CoA production). Ketone and octanoate oxidation was unaffected by hypertrophy. Oxidation of glucose and glycolysis was increased in both models of pressure overload, but the overall contribution of glucose to oxidative energy production remained modest. In contrast, oxidation of lactate and pyruvate was stimulated by hypertrophy to the extent that their metabolism provided most of the energy production even at physiological concentrations. (Service 9) REPORT PERIOD: (09/01/97-08/31/98)

我们继续应用 13C NMR 方法进行代谢分析离体大鼠心脏。去年的一个目标是肥厚心脏组织的代谢分析。早期研究表明从外源脂肪酸或碳水化合物和内源性能量储存可能会大大增加左心室肥厚受到干扰。长的贡献链脂肪酸 (0.35 mM)、乳酸 (1.2 mM)、丙酮酸 (0.09 mM)、葡萄糖 (5.5 mM)、乙酰乙酸盐 (0.12 mM)、α-羟基丁酸盐 (0.05 mM)、通过 13C NMR 测定能源生产的内源性来源两种标准左心室模型的同位素分析肥厚、自发性高血压大鼠和主动脉带状斯普拉格-道利大鼠。来自各种来源的乙酰辅酶A的分数两个对照组之间的来源没有差异：脂肪酸，0.46-0.56；乳酸盐加丙酮酸盐，0.25-0.32；酮类， 0.11-0.12；葡萄糖，0.03-0.05；和内源性来源，0.03-0.06。然而，脂肪酸的氧化显着减少自发性高血压大鼠（乙酰辅酶A产量为0.20 q 0.02，平均 q s.d.）或带状 Sprague-Dawley 大鼠（0.24 q 0.04 乙酰辅酶A的产生）。酮和辛酸氧化不受影响通过肥大。葡萄糖的氧化和糖酵解增加两种压力过载模型，但总体贡献葡萄糖对氧化能的产生仍然适度。相比之下，乳酸和丙酮酸的氧化受到肥大的刺激他们的新陈代谢提供了大部分能量产生即使在生理浓度下。（服务 9）报告期： (09/01/97-08/31/98)