CORE--RADIOIMMUNOASSAY OF NEUROENDOCRINE PEPTIDES AND PROTEINS

核心--神经内分泌肽和蛋白质的放射免疫分析

• 批准号: 6296410
• 负责人: CATHERINE L RIVIER
• 金额: $ 21.48万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6296410
• 关键词: adrenocorticotropic hormone; biomedical facility; corticosterone; corticotropin releasing factor; enzyme linked immunosorbent assay; interleukin 1; interleukin 6; laboratory mouse; laboratory rat; neuropeptides; radioimmunoassay; radiotracer; somatotropin; tumor necrosis factor alpha; vasopressins

Description: (Taken directly from the application). The investigation hypothalamic peptides and immune signals on the release of pituitary peptides and immune signals on the release of pituitary hormones depends biological fluids. We also need to measure levels of the secretagogues fluids. We also need to measure levels of the secretagogues which alter (CRF) and vasopressin (VP). This Core's aim therefore is to provide assays vasopressin (VP). This Core's aim therefore is to provide assays for the factors CRF and VP, as well as corticosterone and cytokines (interleukin- and VP, as well as corticosterone and cytokines (interleukin-1Beta, for ensuring quality control of these assays. To this effect sensitivity, for ensuring quality control of these assays. To this effect consistently monitored. which are consistently monitored. P01DK267419004 Description: (Taken directly from the application). Core C will characterize novel peptides and proteins isolated by the Projects of this Program. In addition, the nature and site of post translational modifications in both novel and recombinant peptides and proteins will be identified. The Core will also perform a variety of services that essential for the Projects of this Program, including the primary structure determination of synthetic peptides, the chemical characterization of recombinant proteins and the synthesis of oligonucleotides. Chemical sequence analysis and mass spectrometry will be used to characterize the peptides and proteins to be studied. The information obtained from chemical analysis and mass spectrometry is not only complementary but synergetic, especially when investigating structural features of proteins. This synergy becomes particularly important when amount of sample is limited such as in the characterization of natural products. As all of these investigations require specialized equipment and well trained personnel they are best centralized in a Core to increase efficiency and to facilitate quality control.

描述：（直接从应用程序中获取）。调查 垂体释放时下丘脑肽和免疫信号 垂体激素释放时的肽和免疫信号取决于 生物流体。 我们还需要衡量秘密的水平 流体。我们还需要衡量会改变的促分子的水平 （CRF）和加压素（VP）。 因此，该核心的目的是提供测定 加压素（VP）。因此，此核心的目的是为 因素CRF和VP，以及皮质酮和细胞因子（白介素 - 和VP，以及皮质酮和细胞因子（白介素1Beta， 确保对这些测定的质量控制。 为了这种效应敏感性， 确保对这些测定的质量控制。为此 一贯监视。 一致监视。 P01DK267419004 描述：（直接从应用程序中获取）。 Core C Will 特征是由新型肽和蛋白质的特征 这个程序。此外，后翻译的性质和地点 新颖和重组肽和蛋白质的修饰将 被识别。核心还将执行各种服务 对于该计划的项目，包括主要的项目至关重要 合成肽的结构测定，化学 重组蛋白的表征和合成 寡核苷酸。化学序列分析和质谱将 用于表征要研究的肽和蛋白质。这 从化学分析和质谱法获得的信息不是 仅互补但协同性，尤其是在调查时 蛋白质的结构特征。这种协同作用特别 当样本量受到限制时，例如在 天然产物的表征。作为所有这些调查 需要专业的设备和训练有素的人员 集中以提高效率并促进质量 控制。