CORE--VIROLOGY

核心--病毒学

• 批准号: 6099254
• 负责人: MICHAEL B. AGY
• 金额: $ 34.34万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099254
• 关键词: AIDS vaccines; Macaca nemestrina; animal tissue; antibody titering; biomedical facility; enzyme linked immunosorbent assay; human immunodeficiency virus 1; immunofluorescence technique; mixed tissue /cell culture; neutralizing antibody; polymerase chain reaction; recombinant virus; simian immunodeficiency virus; virus antigen; western blottings

The Virology Core (Core C) will provide routine laboratory support for Projects 1-4 and the Animal Core (Core A). The Virology Core will work closely with the Nonhuman Primate Core (Core A) and the Molecular Biology Core (Core B) to determine the macaque infectious dose (MID) of several challenge SHIV stocks. Once characterized these stocks will be used to fulfill the specific aims of Projects 1-4. Core C has budgeted for supplies to support Projects 1-4 and Core A. In addition to time lines, individual Project Leaders have provided the Virology Core with detailed lists of routine assays to support their proposed macaque projects. By centralizing laboratory activities in a service core we will minimize supply and reagnt waste as well as duplication of efforts and provide a continuity between projects. The Virology Core is also responsible for the safe operation and maintenance of the biosafety level-3 laboratories, ensuring the most efficient use of this limited laboratory resource.

病毒核心（核心C）将为常规实验室提供支持 项目1-4和动物核心（核心A）。病毒核心将起作用 与非人类灵长类动物核心（核心A）和分子紧密 生物学核心（核心B）确定猕猴感染剂量（中） 几个挑战SHIV股票。 一旦表征了这些股票 用于实现项目1-4的具体目标。 Core C已预算 为了支持项目1-4和核心A的供应。除了时间 单个项目领导者的线路为病毒学核心提供了 详细的例行测定清单，以支持他们提议的猕猴 项目。 通过将实验室活动集中到服务核心中，我们 最小 并提供项目之间的连续性。 病毒学核心也是 负责生物安全的安全操作和维护 3级实验室，确保最有效地利用此限制 实验室资源。