ALCOHOL AND THE PENTOSE CYCLE

酒精和戊糖循环

• 批准号: 3110202
• 负责人: JOSEPH KATZ
• 金额: $ 10.55万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3110202
• 关键词: NAD(H) phosphate; alcoholic beverage consumption; alcoholism /alcohol abuse; liver cells; liver metabolism; malate dehydrogenase; pentose phosphate shunt; radiotracer

The effect of alcohol intake on the operation of the pentose cycle and NADPH generation will be studied in rats in vivo and in vitro. Chronic alcoholic rats will be used when "drunk" (high blood alcohol) and "sober," with control diet substituted overnight for that with ethanol. Methods to calculate the pentose cycle developed by us will be used. For in vivo studies rats will be infused with 2-3H glucose and 1-14C acetate, and glucose isolated. The formation of NADPH will be calculated from the turnover of 2-3H glucose and the distribution of 14C in carbons 1,2 and 3 of glucose. Experiments in vitro will be with isolated hepatocytes from chronic alcoholic "drunk" and "sober" and control rats. The cells will be incubated with 2-3h glucose and 1-14C galactose, and the effect of ethanol on the pathways of glucose-6P and NADPH yield will be determined by novel procedures. The role of NADPH production by malic enzyme will be explored. The source of NADPH for the hydroxylation of aniline and hexobarbitol in alcoholic and control rats will be examined.

酒精摄入对戊糖循环运作的影响 将在大鼠体内和体外研究 NADPH 的生成。 慢性的 酒精老鼠将在“醉酒”（高血液酒精）和“清醒”时使用， 用对照饮食代替乙醇过夜。 方法 计算将使用我们开发的戊糖循环。 对于体内 研究将给老鼠输注 2-3H 葡萄糖和 1-14C 乙酸盐，并且 葡萄糖分离。 NADPH 的形成量按下式计算： 2-3H 葡萄糖的周转以及 14C 在碳 1,2 和 3 中的分布 葡萄糖。 体外实验将使用来自分离的肝细胞 慢性酒精“醉酒”和“清醒”以及对照大鼠。 细胞将是 与葡萄糖和1-14C半乳糖一起孵育2-3小时，以及乙醇的影响 葡萄糖-6P和NADPH产量途径的影响将由新的方法确定 程序。 苹果酸酶产生 NADPH 的作用是 探索过。 苯胺羟基化反应的 NADPH 来源 将检查酒精大鼠和对照大鼠中的己巴比妥。