GENDER AND MENOPAUSE ON NUTRIENT AND ENERGY METABOLISM

性别和更年期对营养和能量代谢的影响

• 批准号: 6165075
• 负责人: TRACY J HORTON
• 金额: $ 2.29万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6165075
• 关键词: bioenergetics; calorimetry; carbohydrate metabolism; catecholamines; clinical research; coronary disorder; dietary lipid; disease /disorder proneness /risk; exercise; free fatty acids; gender difference; hormone regulation /control mechanism; hormone therapy; human subject; lipid metabolism; lipolysis; menopause; muscle metabolism; nutrient bioavailability; nutrition related tag; obesity; sex hormones; stable isotope; triglycerides; very low density lipoprotein

The overall aim of the P.I.'s research is to better understand gender differences in energy and nutrient metabolism that related to the development and/or protection against disease. The health risks associated with a number of diseases including obesity and coronary heart disease are different between males and females. Knowledge of gender differences in energy and nutrient metabolism are required to fully understand the metabolic basis of these diseases. Studies in this proposal will begin to determine gender specific aspects of energy and nutrient metabolism and how these change at menopause. Specific aim number 1 will be to determine the sources of carbohydrate and lipid fuels utilized during exercise in males and females. This will be determined using indirect calorimetry and stable isotope techniques and muscle biopsies. Specific aim number 2 will be to determine the lipolytic response to catecholamines in males and females by using the pancreatic clamp technique in conjunction with catecholamine infusions and stable isotope measurements. Specific aim number 3 will be to determine whether males and females differ in their ability to remove exogenous lipid from the circulation and whether there are gender differences in the ability of insulin to suppress endogenous very-low density lipoprotein triglyceride (VLDL-TG) production. Triglyceride removal will be assessed in response to an intravenous fat tolerance test. Relative changes in VLDL-TG production will be measured following the radioactive labeling of VLDL-TG and measurement of VLDL-TG kinetics under basal and hyperinsulinemic/euglycemic conditions. Specific aim number 4 will be to determine the effect of menopause and hormone replacement therapy on energy expenditure and resting substrate utilization. Free-living energy expenditure will be measured using the doubly labelled water technique and substrate utilization will be assessed using indirect calorimetry and stable isotope techniques. These studies will further our understanding of the metabolic basis for disease risk in males and females.

P.I.研究的总体目的是更好地了解性别 能源和营养代谢的差异与 发育和/或保护疾病。 健康风险 与多种疾病有关，包括肥胖和冠状动脉 男性和女性之间的心脏病不同。知识 需要能量和营养代谢的性别差异才能 充分了解这些疾病的代谢基础。 对此进行了研究 提案将开始确定能源的性别特定方面 营养代谢以及这些新代谢如何变化。 具体目标 数字1将是确定碳水化合物和脂质的来源 在男性和女性运动过程中使用的燃料。 这将是 使用间接量热法和稳定同位素技术确定 肌肉活检。 具体目标2将是确定 通过使用脂肪分解反应对男性和女性的儿茶酚胺的反应 胰腺技术与儿茶酚胺输注 和稳定的同位素测量。特定目标3将是 确定男性和女性的消除能力是否有所不同 循环中的外源脂质以及是否有性别 胰岛素抑制内源性极低的能力的差异 密度脂蛋白甘油三酸酯（VLDL-TG）产生。 甘油三酸酯 将根据静脉脂肪的耐受性评估去除 测试。 VLDL-TG生产的相对变化将遵循 VLDL-TG的放射性标记和VLDL-TG动力学的测量 在基础和高胰岛素/尤利克血糖条件下。 具体目标 数字4将是确定更年期和激素的影响 替代能量消耗和静止基板的疗法 利用率。 自由生活的能源支出将使用 双重标记的水技术和底物利用率将是 使用间接量热法和稳定的同位素技术评估。 这些研究将进一步了解我们对代谢基础的理解 男性和女性的疾病风险。