NUCLEOTIDE INDUCED STRUCTURAL CHANGES IN MYOSINS

核苷酸诱导的肌球蛋白结构变化

• 批准号: 6171625
• 负责人: RONALD A MILLIGAN
• 金额: $ 38.89万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-19 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6171625
• 关键词: actins; adenosine diphosphate; biochemistry; biomechanics; brush border membrane; conformation; electron microscopy; freeze etching; guanosine diphosphate; muscle function; myosins; smooth muscle

DESCRIPTION: (Adapted from abstract) The long term goals of this research are to visualize the conformational changes in the myosin head in both the attached and detached stages of the crossbridge cycle, and to place these observations within the context of mosin mechanochemistry. In the experiments described here, cryo-electron microscopy and image analysis will be used to answer the following fundamental questions: 1)Are the ADP-induced changes seen in smooth muscle myosin and brush border myosin I a charactistic of myosins in general? 2) Are there additional dramatic conformational changes associated with attached states earlier in the cycle? And 3) Is the conformation of the myosin head prior to actin attachment distinct from the ADP.Pi, ADP and rigor attached states? The data to be obtain is designed to establish general principles relating biochemical transitions, conformational changes and function in myosin motors, and pave the way for a more complete understanding of myosin mechanochemistry.

描述：（（根据摘要改编）这项研究的长期目标 要可视化肌球蛋白头的构象变化 连接和独立阶段的Crossbridge循环，并将其放置 在Mosin机械化学的背景下进行观察。 在 此处描述的实验，冷冻电子显微镜和图像分析将会 用于回答以下基本问题：1） ADP引起的变化在平滑肌肌球蛋白和刷子边界肌球蛋白I 一般来说，肌球蛋白的炭化？ 2）还有其他戏剧性 周期早期与附属状态相关的构象变化？ 3）是肌动蛋白附着之前的肌球蛋白头的构象 不同于ADP.PI，ADP和严格的状态？ 数据是 获得的旨在建立与生化有关的一般原则 肌球蛋白电机的过渡，构象变化和功能以及铺路 更完整了解肌球蛋白机械化学的方法。