MECHANISM OF IMMUNOGLOBULIN HYPERMUTATION

免疫球蛋白超突变机制

• 批准号: 6091770
• 负责人: URSULA B STORB
• 金额: $ 42.25万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6091770
• 关键词: B lymphocyte; RNA; genetic regulatory element; human genetic material tag; human subject; immunoglobulin genes; laboratory mouse; nucleic acid structure; tissue /cell culture; transcription factor; transfection

This application is for a new grant to study the mechanism of somatic hypermutation of immunoglobulin (Ig) genes. Our previous work has shown that this process depends on initiation of transcription. The new aims are directed at determining how transcription relates to the somatic mutation process, what the role is of the primary sequence which is the target for mutation and what the cis-acting regulatory elements consist of. Finally, we plan to clone and identify the gene(s) that encode a postulated mutator factor. These studies are important to understand the creation of the lg repertoire with the potential of reacting against any foreign antigen, including tumor antigens. Also, somatic hypermutation has been implicated in autoimmunities. Finally, certain malignant lymphoid tumors arise during the lg gene somatic mutation process and understanding its mechanism will shed light on the tumorigenesis and, hopefully, its prevention.

该应用是为了研究免疫球蛋白（IG）基因的体细胞超名机制的新赠款。我们以前的工作表明，此过程取决于转录的启动。新目标旨在确定转录与体躯体突变过程的关系，主要序列的作用是突变的靶标，以及顺式作用的调节元件由什么组成。最后，我们计划克隆并确定编码假定突变因子的基因。这些研究对于了解LG曲目的创建很重要，具有与任何外国抗原（包括肿瘤抗原）反应的潜力。同样，体育次数也与自身免疫有关。最后，在LG基因体细胞突变过程中出现了某些恶性淋巴肿瘤，并且理解其机制将阐明肿瘤发生，并希望它的预防。