T CELL MEMORY TO RESPIRATORY VIRUSES

呼吸道病毒的 T 细胞记忆

基本信息

批准号：
6169279
负责人：
PETER C DOHERTY
金额：
$ 18.28万
依托单位：
ST. JUDE CHILDREN'S RESEARCH HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-04-01 至 2001-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6169279
关键词：
MHC class II antigen T cell receptor cytotoxic T lymphocyte helper T lymphocyte immunologic memory influenzavirus A laboratory mouse leukocyte activation /transformation parainfluenza virus type 1 tissue /cell culture

项目摘要

Respiratory virus infections ar a major problem for the aged, the very young, and a common complicating factor in immunocompromised transplant or cancer patients. In this context, the practical challenges are to develop better vaccines and enhanced mechanisms for maintaining and manipulating the cell populations that comprise immunological memory. The obverse of this problem is the desire to subvert the consequences of prior antigen priming in, for example, the use of virus-vector systems for gene therapy of cystic fibrosis. An underlying difficulty for both sets of approaches is that immunological memory is, in general, poorly understood. The experimental dissection of memory is technically demanding, rigorous analytical approaches have often been lacking, and there is substantial controversy and confusion. Many of the studies with non-viral systems are of limited value, as the experiments have been done with phenotypically- marked lymphocyte populations of unknown antigen specificity. Considerable effort has thus been made to develop mouse models for the quantitative, functional analysis of T cell memory to a parainfluenza type 1 virus (Sendai) and to the influenza A viruses. The intention is to use these experimental systems for the further clarification of the establishment, maintenance and recall phase of virus-specific CD4+ and CD8+ T cell memory. Attention will be given to the relative significance of events driven via the clonotypic T cell receptor for antigen, and the possible consequences of both intercurrent infections and more physiologically-mediated processes associated with the maintenance of homeostasis in the total pool of T lymphocytes. The experiments will inevitably lead to better understanding of the nature of T cell memory, and help provide novel insights for both vaccine development and possible therapy.

呼吸道病毒感染是老年人的主要问题，年轻，是免疫功能低下的移植或癌症患者。在这种情况下，实际挑战是发展更好的疫苗和增强的维护和操纵机制包括免疫记忆的细胞群体。正面这个问题是渴望颠覆先前抗原的后果例如，启动病毒矢量系统用于基因治疗囊性纤维化。两组方法的根本困难一般来说，免疫记忆是很少了解的。这记忆的实验解剖在技术上是苛刻的，严格的分析方法经常缺乏，并且有实质性的争议和混乱。许多非病毒系统的研究是价值有限，因为实验是通过表型进行的未知抗原特异性的明显淋巴细胞种群。大量因此，已经努力开发用于定量的小鼠模型 T细胞存储器对1型副菌的功能分析（仙台）和流感病毒。目的是使用这些实验系统，以进一步阐明机构，病毒特异性CD4+和CD8+ T细胞存储器的维持和召回阶段。将注意通过驱动的事件的相对意义抗原的clonotypic T细胞受体和可能的后果互动感染和更多生理介导的过程与在T的总池中维持体内平衡有关淋巴细胞。实验将不可避免地提出更好的理解 T细胞记忆的性质，并有助于为两者提供新颖的见解疫苗开发和可能的治疗。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Quantitative analysis of the CD8+ T-cell response to readily eliminated and persistent viruses.

DOI：
10.1098/rstb.2000.0647
发表时间：
2000-08
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
0
作者：
Peter C. Doherty;J. Riberdy;Gabrielle T. Belz
通讯作者：
Peter C. Doherty;J. Riberdy;Gabrielle T. Belz

Kinetic analysis of the specific host response to a murine gammaherpesvirus.

对鼠伽马疱疹病毒的特定宿主反应的动力学分析。