ANTIBODIES TO ISS FOR STUDY OF VIRULENT E. COLI

用于研究强毒大肠杆菌的 ISS 抗体

• 批准号: 6166272
• 负责人: Catherine Mary Logue
• 金额: $ 14.1万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6166272
• 关键词: Aves; Escherichia coli; antiserum; bacterial antigens; complement; monoclonal antibody; protein purification; virulence

DESCRIPTION (Adapted from the applicant's abstract): Colibacillosis is a serious economical problem for the animal production industry. In addition, it is a serious concern for human health because animals are an important source of Escherichia coli strains that can cause severe infections in humans. Data from the PI's laboratory suggest that complement resistance is an important bacterial virulence factor that contributes to the pathogenicity of extraintestinal infections in birds, which are caused by avian isolates of E. coli. This project is design to isolate and produce monoclonal antibodies against Iss, a plasmid-encoded outer membrane protein that contributes to complement resistance by a mechanism that remains to be elucidated. The antibodies will be used to confirm the surface exposure of Iss and examine if the amount of surface-exposed Iss is correlated with the virulence and complement resistance of E. coli avian isolates. This project will be used to introduce and train undergraduate students and strengthen the research program at North Dakota State University.

描述（改编自申请人的摘要）：ColibaCillosis是一个 动物生产行业的严重经济问题。另外，它 是人类健康的严重关注，因为动物是重要的来源 大肠杆菌菌株可能引起人类严重感染。数据 从PI的实验室中表明，补体抵抗是重要的 有助于致病性的细菌毒力因子 鸟类的肠外感染是由E的鸟类分离株引起的。 大肠杆菌。该项目的设计旨在隔离和产生单克隆抗体 反对ISS，质粒编码的外膜蛋白有助于 通过尚待阐明的机制补充抗性。这 抗体将用于确认ISS的表面暴露并检查是否是否 表面暴露IS的量与毒力和 大肠杆菌鸟类分离株的补体抗性。这个项目将用于 介绍和培训本科生并加强研究计划 在北达科他州立大学。