CORE--PREPARATION, STORAGE, AND SHIPPING OF PLANT EXTRACTS AND ASSAYS

核心——植物提取物和检测的制备、储存和运输

• 批准号: 6352742
• 负责人: Sidney Michael Hecht
• 金额: $ 14.83万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352742
• 关键词: DNA damage; DNA directed DNA polymerase; DNA topoisomerases; antineoplastics; chromatography; drug screening /evaluation; enzyme inhibitors; plant extracts; preservation

This Core facility will be responsible for carrying out the preparation, storage and shipping of plant extracts, and much of the assay work run within the NPDDG. This is to include . soaking of plant materials; weighing, packaging, shipping and inventorying of plant extracts . testing of extracts for the presence of DNA damaging agents, DNA topoisomerase inhibitors and DNA polymerase beta inhibitors, in support of Programs 2. . isolation of purified DNA topoisomerase I and DNA polymerase beta for assay work . screening of samples received from NCI natural products repository . guiding the choice of extracts by the development/application of secondary discrimination assays in support of Laboratory Programs 2 . guiding the fractionation of extracts within Laboratory Program 3

该核心设施将负责进行准备工作， 植物提取物的储存和运输以及大部分检测工作的运行 NPDDG 范围内。 这是要包括 。 浸泡植物材料；称重、包装、运输和 植物提取物库存 。 测试提取物中是否存在 DNA 损伤剂、DNA 拓扑异构酶抑制剂和 DNA 聚合酶 β 抑制剂，支持 节目2. 。 分离纯化的 DNA 拓扑异构酶 I 和 DNA 聚合酶 beta 化验工作 。 筛选从 NCI 天然产物储存库收到的样品 。 通过开发/应用来指导提取物的选择 支持实验室计划 2 的二次辨别分析 。 指导实验室计划 3 中提取物的分馏