CORE--LABORATORY FACILITY

核心--实验室设施

• 批准号: 6299634
• 负责人: WALTER E STAMM
• 金额: $ 15.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2000-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299634
• 关键词: Chlamydia trachomatis; Neisseria gonorrhoeae; biomedical facility; clinical research; communicable disease diagnosis; cooperative study; epidemiology; genetic strain; human data; human tissue; nucleic acid sequence; patient /disease registry; polymerase chain reaction; pulsed field gel electrophoresis; serotyping; sexually transmitted diseases

The STD-CRC laboratory core will: 1) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-CRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant clinical and epidemiologic projects within the STD-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a gonorrhea registry to continue assessment of factors associated with reinfection with Neisseria gonorrhoeae. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; PCR and LCR tests for chlamydia; Pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; and Micro IF serology for C. trachomatis. Provision of these and other tests in a central core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. Such availability will foster the use of microbiological endpoints in behavioral studies. While it is not the purpose of the laboratory core to support basic science work, the core will actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. For example, we propose the further development and implementation of a PFGE based typing system for gonococci that will demonstrate heterogeneity even among strains of the same auxotype and serovar, providing a much more powerful tool for demonstrating strain diversity. The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.

STD-CRC实验室核心将：1）提供必要的专业 研究人员的微生物和实验室专业知识 STD-CRC内的临床，流行病学和行为项目； 2） 促进在基础科学或 分子生物学到相关的临床和流行病学项目 STD-CRC； 3）作为国家和国际参考 其他研究性传播疾病的研究人员的实验室； 4）保持 淋病注册中心继续评估与 再感染淋病奈瑟氏菌。提供的实验室专业知识 核心将包括高度专业的实验室测试 即使在其他研究实验室中也不是常规可用，但 拟议的流行病学或临床研究的成功不可或缺。 此类测试的示例包括确定沙眼梭状疱疹血清射手； 淋球菌配体和血清型的测定； PCR和LCR测试 对于衣原体； N.脉冲场凝胶电泳（PFGE）分析。 淋病；选定衣原体菌株的MOMP核苷酸序列；和 微观的微观曲霉学血清学。提供这些测试和其他测试 在中央核心实验室确保它们将由 技术经验丰富的研究人员，降低了规模经济的成本， 并使这些测试可用于进行行为和行为的研究人员 流行病学研究否则可能无法进入实验室 合作者。这种可用性将促进微生物的使用 行为研究中的终点。 虽然不是实验室核心支持基本的目的 科学工作，核心将积极寻求适当的转移 从基础科学实验室到相关临床和 流行病学项目。例如，我们提出进一步的发展 以及针对Gonococci的基于PFGE的打字系统的实施 即使在相同的体型菌株中也表现出异质性 血清，为证明压力提供了更强大的工具 多样性。实验室核心内执行的程序将是 作为其他国家和国际资源可用于其他 调查人员，消除了他们发展昂贵和 技术要求进行测试。