MOLECULAR ORGANIZATION OF LUNG SURFACTANT MONOLAYERS

肺表面活性剂单层的分子组织

• 批准号: 6140056
• 负责人: BARBORA PIKNOVA
• 金额: $ 4.63万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6140056
• 关键词: adult respiratory distress syndrome; biophysics; cholesterol; compression; fluorescence microscopy; intermolecular interaction; molecular assembly /self assembly; molecular film; palmitates; phosphatidylcholines; phospholipids; physical property; pulmonary surfactants; respiratory airway pressure; respiratory distress syndrome of newborn; structural biology; video microscopy

Pulmonary surfactant is defective in the neonatal Respiratory Distress Syndrome (RDS) and in acute RDS that occurs in patients of all ages. The clinical treatment of RDS involves the use of natural or synthetic pulmonary surfactant. However, the mechanism of formation and structure of an active surfactant film at physiological conditions of near-zero surface tension are still relatively unknown. This research proposal aims to understand the structural aspects of an active surfactant film using epifluorescence microscopy on natural as well as model surfactants. The results obtained will help to understand the mechanism by which the stable surfactant films are formed in vivo and to evaluate the respective role of different surfactant components in the formation and stabilization of these films under physiological conditions.

肺表面活性物质在新生儿呼吸窘迫综合征 (RDS) 和各年龄段患者发生的急性 RDS 中存在缺陷。 RDS 的临床治疗涉及使用天然或合成的肺表面活性物质。然而，在接近零表面张力的生理条件下活性表面活性剂膜的形成和结构机制仍然相对未知。该研究计划旨在利用落射荧光显微镜对天然和模型表面活性剂了解活性表面活性剂膜的结构。获得的结果将有助于了解体内稳定的表面活性剂膜形成的机制，并评估不同表面活性剂组分在生理条件下这些膜的形成和稳定中各自的作用。