ALPHA 9 NICOTINIC RECEPTORS OF SACCULAR HAIR CELLS

囊状毛细胞的 ALPHA 9 烟碱受体

• 批准号: 6175590
• 负责人: STEVEN F MYERS
• 金额: $ 1.16万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6175590
• 关键词: DNA; animal genetic material tag; ear hair cell; labyrinth; neurotransmitters; nicotinic receptors; nucleic acid sequence; polymerase chain reaction; protein sequence; trout /salmon

Elucidating the roles played by the auditory and vestibular efferent systems is an important objective of current inner ear neuroscience. The proposed research will contribute to a better understanding of efferent effects on sensory hair cells at the molecular level through analysis of amino acid sequence and functional domains of the alpha9 acetylcholine receptor. The principal objectives of the proposed training period is to obtain a complete DNA sequence of the alpha9 nicotinic acetylcholine receptor from trout saccular hair cells, and to begin functional domain analysis through comparisons with what is known from other species about alpha9 and other alpha-type nicotinic acetylcholine receptors. To make appropriate extrapolations to human health and disease, contemporary research has made use of a wide variety of animal model systems. The trout sacculus provides an advantageous model where a hair cell sheet containing thousands of hair cells can be readily dissected and studied. This model system lends itself particularly well to biochemical and molecular biological questions that require adequate numbers of a single cell type. Functional domain analysis of the alpha9 receptor protein can only be enhanced by knowing the molecular structure of alpha9 receptor proteins from diverse species. Detailed comparisons of alpha9 protein structure across widely separated species should provide a rich source of clues into the molecular bases of alpha9 receptor function.

阐明听觉和前庭传出系统所扮演的角色是当前内耳神经科学的重要目标。 提出的研究将通过分析氨基酸序列和α9乙酰胆碱受体的氨基酸序列和功能结构域来更好地理解分子水平上的感觉毛细胞的影响。 拟议训练期的主要目标是从鳟鱼saccular saccular毛细胞中获得α9烟碱乙酰胆碱受体的完整DNA序列，并通过与其他关于α9和其他α-型烟碱乙酰胆碱受体的知识的比较开始功能结构域分析。 为了对人类健康和疾病进行适当的外推，当代研究利用了各种动物模型系统。 鳟鱼囊提供了一个有利的模型，其中含有成千上万个毛细胞的毛细胞片可以容易解剖和研究。 该模型系统特别适合生化和分子生物学问题，这些问题需要足够数量的单细胞类型。 只能通过了解来自不同物种的α9受体蛋白的分子结构来增强α9受体蛋白的功能结构域分析。广泛分离物种的α9蛋白结构的详细比较应为α9受体功能的分子碱基提供丰富的线索来源。