REGULATION OF BLOOD CELL DEVELOPMENT IN DROSOPHILA

果蝇血细胞发育的调节

• 批准号: 6208601
• 负责人: ASHA HARIKRISHNAN
• 金额: $ 4.43万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6208601
• 关键词: Drosophilidae; JAK kinase; apoptosis; arthropod genetics; biological signal transduction; blood cells; cell differentiation; cell growth regulation; cell proliferation; cellular immunity; developmental genetics; gene expression; genetic regulation; genetic screening; hematopoiesis; hematopoietic stem cells; mitogen activated protein kinase; mutant; phagocytosis; tissue /cell culture

Hemocytes or blood cells play an important role in the cellular immune response of Drosophila and are involved in the phagocytosis of pathogenic microbes and in the encapsulation of larger foreign invaders. Recent studies have highlighted striking similarities between innate immunity in vertebrates and the immune response of insects. The precise mechanism by which distinct types of blood cells are generated in Drosophila is still poorly understood. Our preliminary experiments show that Notch may play a crucial role in cell-fate determination during hematopoiesis. A goal of this proposal is to study the specific function of Notch in blood cell development by using mutant forms of Notch and to test if the Notch and Jak-Stat pathways interact to regulate hematopoiesis in Drosophila. In addition, we propose to conduct a genetic screen to identify genes that play an important role in regulating the proliferation of blood cell precursors and in specifying distinct hematopoietic fates. We anticipate that these studies will provide insights into the genetic pathways that regulate both Drosophila and mammalian hematopoiesis.

血细胞或血细胞在果蝇的细胞免疫反应中起重要作用，并参与致病微生物的吞噬作用以及较大的外国入侵者的封装。 最近的研究强调了脊椎动物先天免疫与昆虫免疫反应之间的惊人相似之处。 在果蝇中产生不同类型的血细胞的确切机制仍然鲜为人知。我们的初步实验表明，Notch在造血过程中可能在细胞命令性测定中起关键作用。 该建议的一个目的是通过使用Notch的突变体形式来研究Notch在血细胞发育中的特定功能，并测试Notch和Jak-Stat途径是否相互作用以调节果蝇中的造血。 此外，我们建议进行遗传筛选，以鉴定在调节血细胞前体的增殖和指定不同造血命运方面起重要作用的基因。 我们预计这些研究将提供有关调节果蝇和哺乳动物造血的遗传途径的见解。