MECHANOELECTRICAL FEEDBACK IN HEART

心脏的机电反馈

• 批准号: 6221112
• 负责人: WILLIAM CRAELIUS
• 金额: $ 0.13万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6221112
• 关键词: biological products; biomedical resource; computers; genetics; statistics /biometry

Induced nitric oxide synthase is known to occur in several tissues during sepsis and inflammation. Activation of the nitric oxide pathway has both deleterious and beneficial consequences. At present, no information is available on the transcriptional regulation of human inducible nitric oxide synthase gene expression by cytokines and endotoxin. Our laboratory has recently cloned the human hepatocyte inducible nitric oxide synthase gene(Geller et al. Proc Natl Acad Sci USA 1993; 90:3491-33495). We will use this cDNA to screen a human genomic DNA library and isolate the promoter region of the human induced nitric oxide synthase gene. The promoter will be sequenced and analyzed for transcriptional factor binding sites to characterize the transcriptional regulation of this important enzyme. It is our hope that the information learned by these studies will contribute significantly to our understanding of transcriptional regulation of gene expression in sepsis and, more import antly, the regulation of induced nitric oxide synthesis in sepsis and inflammation.

已知诱导一氧化氮合酶发生在几个组织中 在败血症和炎症期间。 一氧化氮的激活 途径既有有害和有益的后果。 现在， 没有有关人的转录调节的信息 细胞因子和可诱导一氧化氮合酶基因表达和 内毒素。 我们的实验室最近将人类肝细胞克隆 诱导一氧化氮合酶基因（Geller等人。 美国1993; 90：3491-33495）。 我们将使用此cDNA筛选人 基因组DNA文库并隔离人类的启动子区域 诱导一氧化氮合酶基因。 启动子将进行测序 并分析转录因子结合位点以表征 该重要酶的转录调节。 这是我们的 希望这些研究学到的信息能做出贡献 我们对我们对转录调节的理解 败血症中的基因表达，更像进口的调节 败血症和炎症中诱导一氧化氮合成。