MOLECULAR DYNAMICS OF WATER CHANNELS

水通道的分子动力学

• 批准号: 6282450
• 负责人: JORGE FISCHBARG
• 金额: $ 1.17万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282450
• 关键词: biological products; biomedical resource; computers

All eight genes of the original isolate of wild type A/AA/6/60 influenza virus and of the cold adapted A/AA/6/60 vaccine virus have been cloned, sequenced and subjected to RNAfold analysis. Both viruses are ts and attenuated for ferrets. To complete the study, a wild type of the same virus which is both non-ts and virulent will be sequenced and subjected to RNAfold analysis. Relevance of the RNAfolds to the phenotypes of temperature sensitivity and cold adaption as well as to transport of vRNA out of the nucleus will be explored. The second objective of this series of studies is to compare computer generated RNAfold predictions to experimentally deduced structures. SJCRH has a database of 92 nonstructural gene sequences (the smallest gene--890 bases--of the influenza virus). I propose to study their predicted RNAfolds using the Zuker algorithm on the Y-MP to determine whether the folds of the NS gene will fall into groups corresponding to host of origin and whether co nserved domains necessary for functions such as exodus from the cell nucleus may be identified. I will then compare the computer predicted folds to experimentally deduced structures using mung bean nuclease and psoralen cross-linking studies.

野生型A/AA/6/60的原始分离物的所有八个基因 流感病毒和冷适应的A/AA/6/60疫苗病毒已有 被克隆，测序并进行RNA倍分析。 两个都 病毒是TS，并减弱了雪貂。 为了完成研究， 既非TS and viculent又 测序并进行RNA倍分析。 相关性 对温度灵敏度和冷的表型的RNA折叠 适应以及将VRNA从细胞核中传递出来 探索。 这一系列研究的第二个目标是 比较计算机生成的RNAFold预测与实验 推论结构。 SJCRH具有92个非结构基因的数据库 序列（最小的基因 - 890个碱基 - 流感病毒）。 我 建议使用Zuker算法在上研究其预测的RNAFolds Y-MP确定NS基因的折叠是否会落入 与原点宿主相对应的组以及是否共同域 从细胞核等功能（例如出埃及记）可能是 确定。 然后，我将将预测折叠的计算机比较 实验推导的结构使用绿豆核酸酶和 牛皮岩交联研究。