SEQUENCE ANALYSIS OF ARABIDOPSIS THALIANA MICROTUBULE ASSOCIATED PROTEIN CDNAS

拟南芥微管相关蛋白CDNAS的序列分析

• 批准号: 6282519
• 负责人: MELISSA A MELAN
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282519
• 关键词: biomedical resource; biotechnology; clinical research; clinical trials; growth /development; plants

Microtubule associated proteins (MAPs) are of biological interest because they are important inmaintaining the structural organization and stability of the microtubular cytoskelton. The proposed research is aimed at isolating genes which encode MAPs in higher plants, with a particular emphasis upon the eventual determination of how these proteins function in plant cells. The proteins belonging to one group of mammalian MAPs, which includes MAP2, tau an MAP4, all show a high degree of protein sequence conservation within a carboxy-terminal domain that serves to bind microtubles. We hypothesize that these conserved sequences are also present in plant MAP proteins. Our preliminary data showing hybridization between a cDNA encoding the conserved region of mouse MAP2 and genomic DNA from Arabidopsis thalian supports this hypothesis. We propose to isolate full-length MAP cDNA clones from Arabidopsis. One means of confirming whether or not these cDNAs represent true MAP genes will be to compare their nucleotide and derived amino acid sequences with those of known mammalian MAPs. We propose to use the University of Wisconsin GCG sequence analysis programs for these sequences comparisons. This and other aspects of this project will extend the cellular biological knowledge of the plant cytoskeleton and its functions within the cell. It will provide important information about a group of proteins where there is much speculation but little documentation of their functions during critical times in plant development.

微管相关蛋白 (MAP) 具有生物学意义 因为它们对于维持结构组织很重要 和微管细胞骨架的稳定性。 拟议的研究 旨在分离高等植物中编码 MAP 的基因， 特别强调如何最终确定这些 蛋白质在植物细胞中发挥作用。 属于一组的蛋白质 哺乳动物 MAP（包括 MAP2、tau 和 MAP4）均显示出较高的 羧基末端内蛋白质序列的保守程度 用于结合微管的结构域。 我们假设这些 保守序列也存在于植物 MAP 蛋白中。 我们的 初步数据显示编码 cDNA 之间的杂交 小鼠 MAP2 和拟南芥基因组 DNA 的保守区域 塔利安支持这一假设。 我们建议隔离全长 来自拟南芥的 MAP cDNA 克隆。 确认是否或的一种方法 这些 cDNA 并不代表真正的 MAP 基因，而是要比较它们的 核苷酸和衍生的氨基酸序列与已知的序列 哺乳动物 MAP。 我们建议使用威斯康星大学GCG 用于这些序列比较的序列分析程序。 这个和 该项目的其他方面将扩展细胞生物学 了解植物细胞骨架及其在细胞内的功能。 它将提供有关一组蛋白质的重要​​信息，其中 有很多猜测，但对其功能的记录很少 在植物发育的关键时期。