PHOSPHOLIPID METHYLTRANSFERASE IN S CEREVISAE & SCHIZOSACCHAROMYCES POMBE

酿酒酵母中的磷脂甲基转移酶

• 批准号: 6295161
• 负责人: SUSAN Armstrong HENRY
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295161
• 关键词: biological products; biomedical resource; genetics; lipids; nucleic acids; plants; proteins

Phospholipid(PL) methyltransferase catalyzes the last two sequential methylations in the synthesis of phosphatidylcholine(PC) in the phospholipid biosynthetic pathway in yeast, Saccharomyces cerevisiae. The activity of this pathway in other eukaryotic membranes has been implicated in diverse functions such as a regulator of lipid composition, modifier of membrane fluid properties, and as a transducer of receptor mediated signalling. One objective of the research described in this proposal is to purify and characterize PL methyltransferase from yeast. In addition, the regulation and the mechanism of localization of PL methyltransferase will be studied using biochemical and yeast molecular genetic techniques. The structural gene, OP13, encoding this enzyme in S. cerevisiae has been isolated in the Henry laboratory. Antibodies will be used to develop protocols for purifying the native PL methyltransferase and for analyzing its localization within various compartments of the yeast cell. In addition, the Schizosaccharomyces pombe homologue(CH01) to the S. cerevisiae OP13 gene has also been isolated in the Henry laboratory. An initial characterization of the S. pombe CH01 gene will be undertaken and used in a comparison study of PC biosynthesis and regulation in S. pombe and S. cerevisiae. Hopefully, the research proposed will lead to a better understanding of the function and regulation of the phospholipid methylation pathway in the synthesis of PC in yeast.

磷脂（PL）甲基转移酶催化后两种 磷脂酰胆碱（PC）合成中的顺序甲基化 酵母中的磷脂生物合成途径 酿酒酵母。 该途径在其他真核生物中的活动 膜与调节器等多种功能有关 脂质成分，膜流体特性的修饰剂，作为一个 受体介导的信号传导的传感器。 一个目的 该提案中描述的研究是净化和表征PL 来自酵母的甲基转移酶。 另外，法规和 将研究PL甲基转移酶定位的机理 使用生化和酵母分子遗传技术。 这 结构基因，OP13，编码酿酒酵母中的这种酶已经 在亨利实验室中孤立。 抗体将用于开发 纯化天然PL甲基转移酶的协议和 分析其在酵母各个隔室中的本地化 细胞。 此外，精神分裂症pombe同源物（CH01） 酿酒酵母OP13基因也已在亨利中分离出来 实验室。 S. pombe CH01基因的初始表征 将在PC生物合成的比较研究中进行并使用 和S. pombe和S. cerevisiae中的调节。 希望这项研究 提议的将导致对功能的更好理解和 在合成中调节磷脂甲基化途径 PC在酵母中。