EVOLUTION OF METABOLISM: MICROBIAL GENOMES

新陈代谢的进化：微生物基因组

• 批准号: 6122377
• 负责人: CHRISTIAN FORST
• 金额: $ 1.36万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122377
• 关键词: bacteria; biomedical resource; genetics; genome; microorganism; structural biology; technology /technique

The abundance of genomic information by completely sequenced microbial genomes provides a starting point for new insights in the multi-level organization of organisms and their evolution*. We take the next step, away from annotating genes by functions of expressed proteins, towards a more comprehensive description of metabolic pathways of proteins acting as enzymes processing substrates [64]. By comparing pathogenic microbes with free living organisms the pathogenicity can be related to functions which are missing in autotrophs. H. influencae and E. coli are a well known example for such relationships. Similar results can be reported for free living B. subtilis and pathogenic Streptococci. This study aids in drug design and disease treatment by interfering with host-interaction factors and pathways of pathogens. We also pursue the development of methods to construct phylogenies between organisms on the level of metabolic pathways. By a graph-theoretical approach combined with sequence information, pathways of different organisms are related to each other. First results have been obtained by applying this method on pathways related to electron transfer. This suggests the chimeric origin of archaea and thus an early evolution of such pathways [65]. On sequence level and gene cluster organization a closer relationship to bacteria than to eucarya can be reported. These accomplishments open the door for reconstructing the minimal set of genes, regulatory and metabolic pathways of the universal ancestor of all three kingdoms, archaea, bacteria and eucarya.

通过完全测序的大量基因组信息 微生物基因组为新见解提供了一个起点 多层生物的组织及其进化*。 我们接受 下一步，通过表达的功能远离注释基因 蛋白质，更全面地描述代谢 蛋白质充当酶加工底物的途径[64]。 经过 将致病微生物与自由生物体进行比较 致病性可能与在 自养。 H. Imparea和大肠杆菌是一个众所周知的例子 这样的关系。 可以报​​告免费生活的类似结果 B.枯草和致病性链球菌。 这项研究有助于药物 设计和疾病治疗通过干扰宿主相互作用 病原体的因素和途径。 我们还追求 在生物之间构建系统发育的方法 代谢途径。 通过图理论方法与 序列信息，不同生物的途径与 彼此。 通过应用此方法获得了第一个结果 在与电子传输有关的途径上。 这表明嵌合 古细菌的起源，因此是这种途径的早期演变[65]。 在序列水平和基因群集组织上，建立更紧密的关系 可以报​​告对细菌而不是欧洲菌。 这些成就 打开重建最小基因集，调节性的门 和这三个普遍祖先的代谢途径 王国，古细菌，细菌和桉树。