CALMODULIN TARGET PROTEINS; STRUCTURE FUNCTION RELATIONSHIPS

钙调蛋白靶蛋白；

• 批准号: 6122468
• 负责人: ALLEN R RHOADS
• 金额: --
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122468
• 关键词: Chordata; Invertebrata; Mammalia; animal tissue; biomedical resource; carbohydrates; enzymes; growth /development; minerals; nervous system

The research plan involves examining the different families of calmodulin-dependent enzymes to determine: (1) structural or functional motifs (2) hydrophilicity/hydrophobicity index of putative calmodulin-binding regions; (3) the position of phosphorylation sites or pseudosubstrate domains or the homologous regions relative to the calmodulin-binding domain; (4) searching database for sequences similar to regions associated with calmodulin binding; (5) secondary structure prediction in the vicinity of the calmodulin (CAM) binding site; (6) internal repeated sequences via dot plot; (6) examination of local multiple alignments by dynamic programming procedures. Enzyme sequences to be investigated will include the entire spectrum of CAM target proteins. The spectrum includes: Class 1 target proteins that bind to CAM without associated Ca2+ and have little affinity for Ca2+-CAM; Class 2 proteins that bind CAM strongly in the absence of Ca2+ and are positively activated by Ca2+ ; and Class 3 proteins that have high affinity for the CAM-Ca2+ and weak or no affinity for Class 2 are macrophage NO synthases, lung cyclic 3', 5'-nucleotide phosphodiesterase, bordetella pertussis adenylate cyclase, and glycogen phosphorylase b kinase; and class 3 containing the remaining enzymes and proteins such as calcineurin, myosin light chain kinase, caldesmon, adenylate cyclases, fodrin, IP3 kinase, marcks protein, adducins, protein kinases, Ca2+ ATPase pumps and related proteins.

研究计划涉及检查的不同家庭 钙调蛋白依赖性酶确定：（1）结构或 功能基序（2）推定的亲水性/疏水性指数 钙调蛋白结合区域； （3）磷酸化位点的位置 或相对于 钙调蛋白结合域； （4）搜索数据库以获取序列 类似于与钙调蛋白结合相关的区域； （5）次要 钙调蛋白（CAM）结合附近的结构预测 地点; （6）通过点图内部重复序列； （6）检查 通过动态编程程序进行局部多重对齐。 酶 要研究的序列将包括整个CAM的频谱 靶蛋白。 频谱包括：1类靶蛋白 与CAM结合而无需CA2+，对 Ca2+-cam;在不存在的情况下，将CAM强烈结合的2类蛋白质 Ca2+，并被Ca2+积极激活；和3类蛋白 对CAM-CA2+具有很高的亲和力，并且对班级有弱或没有亲和力 2是巨噬细胞无合酶，肺循环3'，5'-核苷酸 磷酸二酯酶，百日咳腺苷酸环化酶和 糖原磷酸化酶B激酶；和3级包含剩余的 酶和蛋白质，例如钙调蛋白，肌球蛋白轻链激酶， Caldesmon，腺苷酸环化酶，禽类蛋白，IP3激酶，Marcks蛋白， adducins，蛋白激酶，Ca2+ ATPase泵和相关蛋白质。