CHOLESTEROL CRYSTAL FORMATION IN REAL & MODEL BIOMEMBRANES

真实的胆固醇晶体形成

• 批准号: 6220489
• 负责人: GERALD W FEIGENSON
• 金额: $ 2.14万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220489
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

During two beam-time accesses at CHESS, each of 48 hours duration, we conducted feasibility studies with regard to the prospect of data collection on crystals of several complexes of type 1 human 17b-Hydroxysteroid Dehydrogenase (17b-HSD1) and small crystals of other steroidogenic enzymes, such as human Cytochrome P450 aromatase. We were able to record diffraction under cryogenic conditions and establish the feasibility of data collection on several of these crystals. Two partial data sets were collected on the crystals of equilin- and coumestrol-complexes of 17b-HSD1. Despite being small (< 0.2mm) and thin (< 0.05mm), both these crystals diffracted to about 2.5
resolution. Data sets were about 55-75% complete. More beam time is required to complete the data collection. Our regular application for more beam time has recently received a favorable rating, permitting us to continue with the process. Preliminary structure analyses with these data sets have enabled us to locate equilin and coumestrol molecules in the active site of 17b-HSD1. These results are being presented at the upcoming Endocrine Society meeting in New Orleans.

在 CHESS 的两次波束时间访问期间，每次持续时间为 48 小时， 我们对数据的前景进行了可行性研究 1 型人类的几种复合物晶体的集合 17b-羟基类固醇脱氢酶 (17b-HSD1) 和小晶体 其他类固醇生成酶，例如人细胞色素 P450 芳香酶。 我们能够在低温条件下记录衍射并且 确定对其中几个数据收集的可行性 晶体。 在晶体上收集了两个部分数据集 17b-HSD1 的马烯雌酚和香豆雌酚复合物。 尽管很小（< 0.2 毫米）和薄（< 0.05 毫米），这两种晶体衍射至约 2.5 分辨率。 数据集大约完成了 55-75%。 更多光束 完成数据收集需要时间。 我们的常规 最近获得了更多光束时间的申请 评级，使我们能够继续该过程。 初步的 对这些数据集的结构分析使我们能够定位 17b-HSD1 活性位点中的马烯雌酚和香豆雌酚分子。 这些结果将在即将召开的内分泌学会上公布 会议在新奥尔良举行。