LASER MICROSURGERY ON MEIOTIC CRANE FLY SPERMATOCYTES: TELOMERES, KINETOCHORES

鹤蝇精母细胞减数分裂的激光显微手术：端粒、动粒

• 批准号: 6119669
• 负责人: JAMES LAFOUNTIAN
• 金额: $ 0.85万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119669
• 关键词: Mammalia; bacteria; bioimaging /biomedical imaging; biomedical resource; health care; human tissue; infection; microscopy

(Supported by NSF MCB 9808290 to J. LaFountain) This project involves using time-lapse video LM to follow the behavior of chromosome fragments created at various stages of meiosis in living crane fly spermatocytes by laser microsurgery. The laser is used to sever chromosomes into fragments that contain kinetochores, telomeres, or fragments that lack both of these structures. The behavior of these various fragments is then followed in vivo by video LM. The goal is to characterize the non-kinetochore based transport properties of meiotic spindles, and to compare these properties with those already known for plants and animal somatic cells. Preliminary data on this project that was obtained by Dr. LaFountains during his visits in 1997 has been used to obtain NSF support for an expanded effort. As a result he will be spending the next 18 months at the BMIRR facility, on sabbatical leave, pursuing the specific aim of his grant most of which involve laser microsurg ery on cr ane fly spermatocytes. Obviously, this work must be conducted at our facility because we have the only DIC based laser microsurgery system in the Nation. Last year this work was abstracted as: LaFountain, J. R., R.W. Cole and C. L. Rieder. (1997) Laser microsurgery of anaphase chromosomes in crane-fly spermatocytes: Kinetochore-independent movement of telomere-containing chromosome fragments. Molec. Biol. Cell 8:171a.

（由 NSF MCB 9808290 J. LaFountain 支持）该项目 涉及使用延时视频 LM 来跟踪 生物体减数分裂各个阶段产生的染色体片段 通过激光显微手术获得鹤蝇精母细胞。 激光用于 将染色体切割成含有着丝粒、端粒的片段， 或缺乏这两种结构的片段。 的行为 这些不同的片段之后是体内的视频 LM。 这 目标是表征基于非着丝粒的传输特性 减数分裂纺锤体的特性，并将这些特性与 已知植物和动物体细胞。 初步数据 LaFountains 博士在访问期间获得的这个项目 1997 年已被用来获得 NSF 对扩大工作的支持。 因此，他将在 BMIRR 度过接下来的 18 个月 设施，正在休假，追求他的补助金的具体目标 其中大部分涉及对鹤蝇精母细胞进行激光显微手术。 显然，这项工作必须在我们的工厂进行，因为我们有 国内唯一基于 DIC 的激光显微手术系统。 去年 这项工作的摘要为：LaFountain、J. R.、R.W. Cole 和 C. L. 里德。 (1997) 后期染色体激光显微手术 鹤蝇精母细胞：不依赖动粒的运动 含有端粒的染色体片段。 莫莱克。 生物。 细胞 8：171a。