FACSTAR PLUS UPGRADE--TURBOSORTING UV LASER WORKSTATION

FACSTAR PLUS升级版--涡轮分选紫外激光工作站

• 批准号: 2503107
• 负责人: DAVID H BOLDT
• 金额: $ 13.85万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503107
• 关键词: biomedical equipment; biomedical equipment purchase; computer program /software; computer system hardware; flow cytometry

Funding is requested for a set of upgrades to the fluorescence activated cell sorter (FACS) in the Institutional Flow Cytometry Laboratory at the University of Texas Health Science Center at San Antonio. The present instrument in this laboratory is a Becton-Dickinson FACStar Plus purchased in 1985 with an NCRR Shared Instrumentation Grant. This instrument has supported a productive and diverse group of NIH-funded investigators; the major user of group of 11 investigators holds 1.9 million dollars per annum in NIH funding accounting for 83 percent of instrument utilization. However, the instrument now is in need of major upgrading to meet the continuing needs of these investigators. The upgrades requested include: (1) addition of turbosorting-permitting sort rates up to 10,000 events per second with 99 percent purity (presently maximal sort rates are 2000 events per second with approximately 95 percent purity); (2) addition of a third laser with both uv-and 488-nm capabilities (presently the instrument lacks a uv-laser and both present lasers have logged greater than 5,000 hours of use); (3) a computer work station to provide for simultaneous analysis of previously stored data while samples for FACS analysis or sorting are being run (not now possible in the facility); and (4) new cell sorting and analytical software (current software is first generation and significantly outdated in terms of precision, flexibility, and efficiency). The FACS facility has been a fundamental core resource at UTHSCSA for 14 years. The requested upgrades will permit this facility to continue to provide availability of state-of-the-art flow cytometric analysis and sorting to the UTHSCSA community.

需要资金对荧光激活进行一系列升级 流式细胞术实验室的细胞分选仪 (FACS) 德克萨斯大学圣安东尼奥健康科学中心。 现在的 该实验室的仪器是 Becton-Dickinson FACStar Plus 1985 年通过 NCRR 共享仪器补助金购买。 这 该仪器支持了 NIH 资助的富有成效且多样化的群体 调查员； 11名调查员组的主要用户持有1.9 每年 100 万美元的 NIH 资助占 83% 仪器利用率。 然而，该仪器现在需要重大 升级以满足这些研究人员的持续需求。这 请求的升级包括：（1）添加允许涡轮排序的排序 速率高达每秒 10,000 个事件，纯度为 99%（目前 最大排序率为每秒 2000 个事件，其中大约 95 个事件 纯度百分比）； (2) 添加第三个同时具有紫外和 488 nm 的激光 功能（目前该仪器缺少紫外激光器，并且都存在 激光器的使用时间已超过 5,000 小时）； (3)计算机工作 站提供对先前存储的数据的同步分析 当样品进行 FACS 分析或排序时（不是现在） 可能在设施内）； (4) 新的细胞分选和分析 软件（当前软件是第一代，并且显着 在精度、灵活性和效率方面已经过时）。 流式细胞仪 14 年来，该设施一直是 UTHSCSA 的基本核心资源。 所请求的升级将使该设施能够继续提供 提供最先进的流式细胞术分析和分选 致 UTHSCSA 社区。