MOLECULAR GENETICS OF EARLY EYE DEVELOPMENT

早期眼睛发育的分子遗传学

• 批准号: 6106863
• 负责人: Stanislav I Tomarev
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6106863
• 关键词: DNA binding protein; animal genetic material tag; cell differentiation; developmental genetics; eye; genetic mapping; homeobox genes; human genetic material tag; immunocytochemistry; in situ hybridization; molecular genetics; zebrafish

The role of homeobox gene, Prox 1 in vertebrate eye development and regeneration was studied. The zebrafish Prox 1 gene was isolated and characterized and its expression pattern was studied by whole-mount immunohistochemistry and in situ hybridization. Expression of Prox 1 was first detected in the ten-somite stage embryos in the ectoderm overlaying the eye buds. As embryogenesis proceeds, Prox 1 expression was also detected in muscle pioneer cells, forebrain-midbrain boundary, ventral hindbrain, and selected cells of the ventral spinal cord. A partial Prox 1 cDNA was isolated from the newt. In the process of lens regeneration in the newt, Prox 1 protein was found to be selectively expressed in the regenerating competent cells of the dorsal iris but not in the cells of ventral iris.

同源基因的作用，Prox 1在脊椎动物眼中 研究了发展和再生。斑马鱼Prox 1 基因被分离并表征，其表达模式为 由整个安装免疫组织化学和原位研究 杂交。首先在 覆盖眼芽的外胚层中的十粒阶段胚胎。 随着胚胎发生的进行，还检测到Prox 1的表达 肌肉先驱细胞，前脑 - 脑边界，腹侧 后脑和腹侧脊髓的选定细胞。部分 从NEWT分离出Prox 1 cDNA。在镜头的过程中 发现Newt的再生，Prox 1蛋白有选择性 在背虹膜的再生能力细胞中表达，但 不在腹虹膜细胞中。