SALIVARY ARGININE/LYSINE/PEPTIDES AND CARIES EXPERIENCE

唾液精氨酸/赖氨酸/肽与龋齿的经验

• 批准号: 6270345
• 负责人: Lawrence A. Tabak
• 金额: $ 15.78万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6270345
• 关键词: acidity /alkalinity; arginine; dental caries; dental caries inhibitor; dental plaque; human subject; immunologic assay /test; lysine; microorganism metabolism; oligopeptides; oral bacteria; saliva

The most consistent difference observed between caries-free and caries- susceptible subjects relates to regulation of plaque pH; caries free individuals neutralize plaque acid more effectively. We hypothesize that this difference is related to elevated levels of free dibasic amino acids and arginine/lysine-containing peptides in the saliva of caries-free persons. We will first quantitate levels of individual low molecular weight peptides in parotid saliva collected from mature caries-free and caries- susceptible adults (equal to or more than 50 yrs.). Salivary peptides which are observed to be associated with caries experience will be characterized for their ability to buffer plaque fluid and to yield ammonia when metabolized by oral microorganisms. We will also raise antibodies to the salivary peptides which are associated with caries experience for the purpose of developing immunoassays to quantitate these peptides. We will then determine the levels of these peptides in a group of children who will be examined longitudinally for four years; at each exam, caries status (i.e. caries-free or caries-susceptible) will be determined and parotid salivary peptides and free amino adds quantitated. The identification of salivary factors which are involved in plaque pH regulation will enhance our understanding of host resistance to dental caries.

无龋齿和龋齿之间观察到的最一致的差异 - 易感受试者与斑块pH的调节有关；龋齿免费 个体更有效地中和斑块酸。我们假设这一点 这种差异与自由二元氨基酸水平升高有关 和精氨酸/含赖氨酸的肽中的唾液中的肽 人。 我们将首先定量单个低分子量的水平 从成熟的龋齿和龋齿收集的腮腺唾液中的肽 - 易感成年人（等于或超过50年）。唾液肽 观察到与龋齿经验有关的 特征以缓冲牌匾流体并产生的能力 通过口服微生物代谢时的氨。我们还将提高 与龋齿相关的唾液肽的抗体 为了开发免疫测定的目的，经验来量化这些 肽。然后，我们将确定一组中这些肽的水平 在纵向检查四年的孩子中；每个 考试，龋齿状态（即无龋齿或龋齿敏感）将是 确定和腮腺唾液肽和游离氨基添加了定量。 识别斑块pH中涉及的唾液因子 法规将增强我们对宿主对牙齿抵抗的理解 龋齿。