CORE--LIVE CELL IMAGING AND FLUORESCENT BIOSENSOR PRODUCTION

核心——活细胞成像和荧光生物传感器生产

基本信息

批准号：
6269605
负责人：
KLAUS HAHN
金额：
$ 21.23万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-07-17 至 1999-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6269605
关键词：
biomedical facility fluorescent dye /probe ionophores ligands protein localization protein structure tissue /cell culture

项目摘要

New approaches in microscopy and fluorescent analog cytochemistry have enabled the trafficking of fluorescent molecules to be studied in real time within living cells (1). The value of such approaches have been greatly extended by producing protein analogs which report changes in their structure or ligand binding through changes in their fluorescence spectrum. This has been accomplished through site-specific protein labeling with environmentally sensitive fluorophores that report changes in their solvent environment, or through labeling with pairs of dyes undergoing fluorescence resonance energy transfer. Using such fluorescent protein biosensors, the spatio-temporal dynamics of protein localization have been correlated with alterations in protein-protein interaction and conformation. This core will supply the resources and expertise to carry out quantitative studies of fluorescent molecules in living cells. Well characterized fluorescent molecules suitable for examining trafficking will be supplied, as well as fluorophores and help for generating novel fluorescent analogs. The core director is actively involved in development of protein analogs which report conformation changes and ligand interactions in vivo (2). The core will provide the novel dyes required for such studies and assist investigators in producing new conformationally-sensitive protein biosensors.

显微镜和荧光模拟细胞化学的新方法已经使荧光分子的运输得以真实研究活细胞内的时间 (1)。这些方法的价值在于通过生产报告变化的蛋白质类似物大大扩展了它们的结构或配体通过荧光的变化结合光谱。这是通过位点特异性蛋白质实现的使用报告变化的环境敏感荧光团进行标记在其溶剂环境中，或通过用成对染料进行标记进行荧光共振能量转移。使用这种荧光蛋白质生物传感器，蛋白质定位的时空动力学与蛋白质-蛋白质相互作用的改变相关构象。该核心将提供资源和专业知识来开展活细胞中荧光分子的定量研究。出色地适合检查贩运的特征性荧光分子将提供，以及荧光团并帮助生成新的荧光类似物。核心董事积极参与开发报告构象变化和配体的蛋白质类似物体内相互作用(2)。核心将提供所需的新型染料进行此类研究并协助研究人员产生新的构象敏感的蛋白质生物传感器。