Affordable near-patient diagnostics to distinguish infectious diseases in the Philippines (AND2ID in Ph)

菲律宾提供经济实惠的近距离患者诊断来区分传染病（Ph 中的 AND2ID）

• 批准号: MR/R025444/1
• 负责人: Elizabeth Hall
• 金额: $ 56.58万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FR025444%2F1
• 关键词: Affordable; near; patient; diagnostics; distinguish

Acute fever may be caused by a range of pathogens, but clinical symptoms may be too non-specific to differentiate the causative organism, so correct diagnosis requires pathogen-specific diagnostic tests. These diagnostics are too expensive for routine use in resource-limited settings. This means that many patients are treated empirically with broad spectrum antibiotics, which may be unecessary, toxic, and increase the risk of antimicrobial resistance. Conversely delayed diagnosis and treatment may lead to poor outcomes. A barrier to low-cost diagnostics in the Philippines, arises from a value chain that spans the world, without Purchasing Power Parity (PPP). If we could use technologies that can be manufactured locally, using local resources, then we have the first step to providing affordable diagnostics in resource poor areas, and delivering a sustained improvement in healthcare, while also developing the local economy. We have made an enzyme (BOON-enzyme) that can do these tests and can be produced almost anywhere without special facilities. We have made the enzyme pink, so that you can see that it has been produced and we have a way of making it stick to sand so that it is very stable. We are going to use one of these enzymes - BOON-Taq in polymerase chain reaction (PCR) - to perform a clinical study in patients with suspected dengue and leptospirosis at the University of Santo Tomas in Manila, to assess the impact of the use of diagnostics on the patient pathway and the disease burden. As a result of these trials we will design a diagnostic kit that can be taken out to rural clinics and we will undertake clinical trials in such a clinic. We will support the study by developing a healthcare economics model of the impact of diagnostics on the patient pathway.

急性发热可能由多种病原体引起，但临床症状可能过于非特异性，无法区分致病微生物，因此正确的诊断需要病原体特异性诊断测试。这些诊断对于资源有限的环境中的常规使用来说过于昂贵。这意味着许多患者根据经验使用广谱抗生素进行治疗，这可能是不必要的、有毒的，并且会增加抗菌素耐药性的风险。相反，延误诊断和治疗可能会导致不良结果。菲律宾低成本诊断的障碍来自于跨越世界的价值链，没有购买力平价（PPP）。如果我们能够使用可以在当地制造、利用当地资源的技术，那么我们就迈出了在资源匮乏地区提供负担得起的诊断服务、持续改善医疗保健的第一步，同时也发展了当地经济。我们已经制造了一种可以进行这些测试的酶（BOON-enzyme），并且几乎可以在任何地方生产，无需特殊设施。我们把酶做成了粉红色，这样你就可以看到它已经生产出来了，我们有办法让它粘在沙子上，这样它就非常稳定。我们将在马尼拉圣托马斯大学使用其中一种酶——聚合酶链式反应 (PCR) 中的 BOON-Taq——对疑似登革热和钩端螺旋体病的患者进行临床研究，以评估使用对患者途径和疾病负担的诊断。根据这些试验的结果，我们将设计一种可以带到农村诊所的诊断试剂盒，并在这样的诊所进行临床试验。我们将通过开发诊断对患者路径影响的医疗经济学模型来支持这项研究。

项目成果

Electrochemically induced in vitro focal hypoxia in human neurons.

• DOI: 10.3389/fcell.2022.968341
• 发表时间: 2022
• 期刊: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
• 影响因子: 5.5
• 作者: Wong, Joseph J. Y.;Varga, Balazs V.;Karadottir, Ragnhildur Thora;Hall, Elizabeth A. H.
• 通讯作者: Hall, Elizabeth A. H.

Electrochemically induced in vitro focal hypoxia in human neurons

电化学诱导人神经元体外局灶性缺氧

• DOI: 10.17863/cam.88711
• 发表时间: 2022
• 作者: Wong J