RAPID PHENOTYPING OF PLASTICITY IN VISUAL CORTEX

视觉皮层可塑性的快速表型

• 批准号: 6076055
• 负责人: MICHAEL P STRYKER
• 金额: $ 21.31万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6076055
• 关键词: electrodes; electroencephalography; evoked potentials; genetic strain; genetically modified animals; handbook; inbreeding; information dissemination; laboratory mouse; neural plasticity; neurogenesis; neurogenetics; neurons; phenotype; rapid diagnosis; retina; vision tests; visual cortex; visual stimulus; visual threshold

The visual system of the mouse offers the opportunity for the use of genetic approaches to an understanding of central nervous system development, function, and pathology. The mouse visual system has many features in common with that of the human and other higher mammals. The present proposal in response to RFA MH-99-006 is for the development, verification, and dissemination of procedures for a quantitative, rapid and reliable assessment of visual function in the mouse that would be sensitive to abnormalities from the level of the optic media and retina up to and including the level of the primary visual cortex. Swept visual evoked potentials recorded over the visual cortex will be used to define the visibility of grating stimuli of a range of contrasts and spatial frequencies. Signal-to-noise ratios in normal mice are sufficiently large to allow reliable measurement of vision through one eye in 240 sec of data collection, consistent with a goal of screening vision through both eyes at a rate of 4 mice/hour. This proposal represents a collaboration between a laboratory that has over the past several years delineated mechanisms of activity-dependent plasticity in the mouse primary visual cortex, along with genetic disturbances in those mechanisms, and one that perfected the swept VEP and distributed it widely for uses including the screening of visual development in human infants. These assays should permit the investigation of the genetic bases of visual system development and function at levels from the eye to the cortex.

小鼠的视觉系统为使用遗传方法提供了对中枢神经系统发展，功能和病理的理解的机会。 小鼠视觉系统具有许多与人类和其他高级哺乳动物的特征。 本提案响应RFA MH-99-006是用于开发，验证和传播程序，以对小鼠中视觉功能进行定量，快速和可靠的视觉功能评估，这将对视觉介质和视网膜水平的异常敏感，直至和包括主要视觉皮层的水平。 在视觉皮层上记录的视觉诱发电位将用于定义一系列对比度和空间频率的光栅刺激的可见性。 正常小鼠中的信噪比足够大，可以通过240秒收集数据收集的一只眼睛来可靠地测量视力，这与以每小时4小鼠的速度筛选视觉的目标一致。 该提案代表了一个实验室之间的合作，该实验室在过去的几年中，在小鼠主要视觉皮层中描述了活性依赖性可塑性的机制，以及这些机制中的遗传障碍，以及一种完善了VEP的遗传性障碍，并将其广泛分配给了它，以便使用它，包括人类无视筛查人类无视的使用。 这些测定应允许研究视觉系统开发和功能从眼睛到皮质的水平的遗传基础。