FUNCTIONAL ANALYSIS OF RBAP2

RBAP2的功能分析

• 批准号: 2895194
• 负责人: WILLIAM R SELLERS
• 金额: $ 9.21万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2895194
• 关键词: DNA binding protein; binding proteins; cell cycle; cell growth regulation; flow cytometry; molecular cloning; phosphoproteins; polymerase chain reaction; protein structure function; protooncogene; retinoblastoma protein; transcription factor; tumor suppressor genes

We have cloned a protein, RBAP2, based upon its ability to bind to the retinoblastoma gene product in vitro. We have extended the clone to full- length status, shown that it is a nuclear phospho- protein, and that it binds to RB in vivo. This binding follows the same genetics as has been established for the interaction of RB and the viral oncoproteins large T antigen, E1A, and E7. This grant now proposes to investigate the biochemical and biological functions of RBAP2 and, in addition, the role of RBAP2 in RB mediated cell-cycle regulation.

我们基于其结合的能力来克隆蛋白质RBAP2 视网膜母细胞瘤基因产物体外。我们已经将克隆扩展到了 长度状态，表明它是一种核磷酸蛋白，并且 结合体内RB。这种结合遵循与以前相同的遗传学 建立用于RB和病毒癌蛋白大的相互作用 T抗原，E1A和E7。该赠款现在建议调查 RBAP2的生化和生物学功能，此外， Rb介导的细胞周期调节中的RBAP2。