TRANSMISSION AND GENETICS OF ALCOHOL DISORDERS IN A NATIVE AMERICAN TRIBE

美洲原住民部落中酒精疾病的传播和遗传学

• 批准号: 6288631
• 负责人: Jeffrey C. LONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288631
• 关键词: Native Americans; alcoholism /alcohol abuse; clinical research; family genetics; gene environment interaction; genetic polymorphism; genetic susceptibility; genome; genotype; human subject; interview; linkage mapping; mental health epidemiology; nucleic acid repetitive sequence

Using a whole genome scan, we will assess genetic linkage to alcoholism and associated psychiatric disorders in Choctaw American Indians. Choctaw is a large Eastern North American Indian tribe with over 30,000 enrolled members living within tribal boundaries in Oklahoma. By contrast to neighboring American Indian tribes that have high prevalences of alcoholism, this tribe stands out because alcoholism has a low prevalence, about 1% and 10% of females and males, respectively. By studying American Indians in the context low alcoholsim, we can expect to reveal different insights into the roles of genetic and/or environmental determinants of alcoholism. Genetic analysis is to be conducted using three samples from the tribe: a small random sample (N=100), 3 large extended families (N>80 per family), and a sample of more than 150 admixed nuclear families. The admixed nuclear familes will be selected to have some Euro-american ancestry and at least one alcoholic family member. The transmission/disequilibrium test (TDT) is the linkage analysis method of principal interest, because it has been shown to have increased power with population admixture. However, the sampling design will also accomodate standard non-parametric two- and multi-point linkage methods. In order to perform the analyses outlined above, individual psychiatric interviews and blood samples have been and continue to be collected. Research diagnoses are made from the psychiatric interviews and DNA for genotyping is being extracted from the blood samples. For the linkage analysis, we expect to type up to 2000 unique sequence DNA polymorphisms spanning the entire human genome as the technology becomes available. In the past year, collection of the random sample and extended families has been completed. Database checks and epidemiological analyses are now under way. - gene mapping (human), neurosciences, drinking patterns/causes, molecular genetics - Human Subjects: Interview, Questionaires, or Surveys Only

使用整个基因组扫描，我们将评估Choctaw American印第安人中酒精中毒和相关精神疾病的遗传联系。乔克托（Choctaw）是一个北美东部的大型印第安人部落，有30,000多名居住在俄克拉荷马州部落边界的入学成员。与邻近的美洲印第安部落相比，酗酒率很高的是，该部落脱颖而出，因为酒精中毒的患病率较低，分别为1％和10％的女性和男性。通过研究美国印第安人在低酒精中，我们可以期望揭示对酒精中毒的遗传和/或环境决定因素的作用的不同见解。遗传分析将使用来自部落的三个样本进行：一个小的随机样本（n = 100），3个大型家庭（每个家庭n> 80）和150多个混合核心家庭的样本。掺杂的核家族将被选为有一些欧美血统和至少一个酒精家庭成员。传输/不平衡测试（TDT）是主要感兴趣的连锁分析方法，因为已证明它随着人口混合而增加了功率。但是，采样设计还将适应标准的非参数两种链接方法。为了执行上面概述的分析，已经并继续收集了个人的精神访谈和血液样本。研究诊断是由精神病学访谈进行的，从血液样本中提取了基因分型的DNA。对于链接分析，我们预计随着技术的可用，最多可以输入跨越整个人类基因组的独特序列DNA多态性。在过去的一年中，随机样本和大家庭的收集已经完成。数据库检查和流行病学分析现在正在进行中。 - 基因映射（人），神经科学，饮酒模式/原因，分子遗传学 - 人类受试者：访谈，问题或调查