Partnership against Biofilm-associated Expression, Acquisition and Transmission of AMR

合作对抗生物膜相关的 AMR 表达、获取和传播

• 批准号: MR/R005621/1
• 负责人: Jeremy Webb
• 金额: $ 38.63万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FR005621%2F1
• 关键词: Partnership; against; Biofilm; associated; Expression

A relatively recent advance in microbiology is the finding that the majority of infections are caused by bacterial biofilms. Biofilms are structured communities of bacteria found on surfaces that become embedded within a self-produced extracellular polymeric matrix. Biofilms can form on tissues or on biomedical surfaces, such as blood catheters or implants, where they act as a reservoir of potential healthcare-associated infection. Bacteria living in biofilms can tolerate much higher antibiotic concentrations compared to planktonic bacteria and survive long enough to evolve antimicrobial resistance (AMR). They form persistent, hard-to-treat infections and exhibit an intrinsic biology that promotes the development and transmission of AMR. The goal of our consortium is to determine how bacteria adapt to antimicrobials during biofilm formation on surfaces coated with antimicrobials, how AMR mutations are acquired and evolve within mature biofilms, and how population dynamics within biofilms affect the transmission of AMR. We address the hypothesis that understanding the contribution of biofilms to AMR acquisition and spread will lead to the development of novelantimicrobial strategies and medical devices that are more effective in preventing biofilm-associated infection and AMR. Our team provides facilities and clinical research governance for experimental and translational medicine. Our synergy of leading laboratory, clinical and translational research across Europe will ensure the best chance to develop novel and successful interventions and therapeutic outcomes.

微生物学的一个相对较新的进展是发现大多数感染是由细菌生物膜引起的。生物膜是表面上发现的结构化细菌群落，嵌入自产的细胞外聚合物基质中。生物膜可以在组织或生物医学表面形成，例如血液导管或植入物，它们充当潜在的医疗保健相关感染的储存库。与浮游细菌相比，生活在生物膜中的细菌可以耐受更高的抗生素浓度，并且可以存活足够长的时间以形成抗菌素耐药性（AMR）。它们形成持久的、难以治疗的感染，并表现出促进抗菌素耐药性发展和传播的内在生物学特性。我们联盟的目标是确定细菌在涂有抗菌药物的表面形成生物膜期间如何适应抗菌药物，AMR 突变如何在成熟的生物膜内获得和进化，以及生物膜内的种群动态如何影响 AMR 的传播。我们提出这样一个假设：了解生物膜对 AMR 获得和传播的贡献将有助于开发新的抗菌策略和医疗设备，更有效地预防生物膜相关感染和 AMR。我们的团队为实验和转化医学提供设施和临床研究管理。我们在欧洲领先的实验室、临床和转化研究的协同作用将确保开发新颖且成功的干预措施和治疗结果的最佳机会。

项目成果

An integrated model system to gain mechanistic insights into biofilm-associated antimicrobial resistance in Pseudomonas aeruginosa MPAO1.

• DOI: 10.1038/s41522-020-00154-8
• 发表时间: 2020-10-30
• 期刊: NPJ biofilms and microbiomes
• 影响因子: 9.2
• 作者: Varadarajan AR;Allan RN;Valentin JDP;Castañeda Ocampo OE;Somerville V;Pietsch F;Buhmann MT;West J;Skipp PJ;van der Mei HC;Ren Q;Schreiber F;Webb JS;Ahrens CH
• 通讯作者: Ahrens CH

An integrated model system to gain mechanistic insights into biofilm formation and antimicrobial resistance development in Pseudomonas aeruginosa MPAO1

• DOI: 10.1101/2020.02.06.936690
• 发表时间: 2020-02
• 期刊: bioRxiv
• 作者: A. Varadarajan;R. Allan;Jules D. P. Valentin;Olga E. Castañeda Ocampo;Vincent Somerville;Franziska Pietsch;Matthias T. Buhmann;Jonathan J. West;P. Skipp;H. C. van der Mei;Q. Ren;F. Schreiber;J. Webb;C. Ahrens

Identification of Potential Antimicrobial Targets of Pseudomonas aeruginosa Biofilms through a Novel Screening Approach.

• DOI: 10.1128/spectrum.03099-22
• 发表时间: 2023-02-13
• 期刊: Microbiology spectrum
• 影响因子: 3.7

Role of the flagellar hook in the structural development and antibiotic tolerance of Pseudomonas aeruginosa biofilms.

• DOI: 10.1038/s41396-021-01157-9
• 发表时间: 2022-04
• 期刊: The ISME journal
• 作者: Valentin JDP;Straub H;Pietsch F;Lemare M;Ahrens CH;Schreiber F;Webb JS;van der Mei HC;Ren Q
• 通讯作者: Ren Q