CORE--CELL BANK AND HLA CLINICAL DATA BASE

核心--细胞库和HLA临床数据库

• 批准号: 3769756
• 负责人: JOHN A HANSEN
• 金额: --
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3769756
• 关键词: biomedical facility; cell bank /registry; data collection; histocompatibility gene; human genetic material tag; human tissue; statistical service /center

The goal of this project is to maintain a cell bank and DNA repository for marrow transplant patients and unrelated donors and maintain a detailed HLA data base incorporating the molecular data that is not routinely reported by the clinical typing laboratory. The research objective is to determine the clinical relevance of matching for class I HLA-A, B, C molecular variants and the new class I HLA-E, F and G genes. The core component of this project will be responsible for accumulating cells and DNA from patients and unrelated donors, and generating as needed EBV-transformed B-lymphoblastoid cell lines (LCL) for special studies. Research data will be collected and integrated with the Division of Clinical Research's Clinical Marrow Transplant Data Base. As data are acquired to define the polymorphism for MHC class I and class II loci, appropriate statistical analysis will be undertaken periodically to assess whether the prevalence of disparity for any given locus is sufficient to allow calculations of informative clinical correlations. The eventual clinical analysis will be performed with the combined data using multivariate methods with the goals of determining the impact of HLA incompatibility on graft rejection and the incidence and severity of GVHD.

该项目的目标是维护细胞库和 DNA 存储库 对于骨髓移植患者和无关的捐赠者，并保持 详细的 HLA 数据库包含未包含的分子数据 由临床分型实验室定期报告。研究 目的是确定 I 类匹配的临床相关性 HLA-A、B、C 分子变异体和新的 I 类 HLA-E、F 和 G 基因。 该项目的核心组件将负责积累 来自患者和无关捐赠者的细胞和 DNA，并生成 需要 EBV 转化的 B 类淋巴母细胞系 (LCL) 研究。研究数据将被收集并与 临床研究部的临床骨髓移植数据库。作为 获取数据来定义 MHC I 类和 I 类的多态性 II 位点，将进行适当的统计分析 定期评估任何给定的差异是否普遍存在 位点足以允许计算信息丰富的临床 相关性。最终的临床分析将通过 使用多变量方法组合数据，目的是确定 HLA不相容性对移植物排斥反应及其发生率的影响 和 GVHD 的严重程度。