FIBER OPTIC SENSOR FOR ENVIRONMENTAL PSEUDOESTROGENS

用于环境假雌激素的光纤传感器

• 批准号: 2882837
• 负责人: Judith L. Erb
• 金额: $ 32.28万
• 依托单位: IA, INC.
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-20 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2882837
• 关键词: bioassay; biomedical equipment development; biosensor device; environmental toxicology; estrogen analog; estrogen receptors; fiber optics; receptor binding

DESCRIPTION (Adapted from applicant's abstract): In response to increasing awareness of the suspected role of estrogen mimics in reproductive disorders and cancer, during Phase I work, the investigators demonstrated the feasibility of developing a fiber optic sensor for screening estrogen mimics. The instrument reports rapid real-time data on binding between biological macromolecules and their ligands. Sensor measurements performed on several estrogens and mimics were well correlated with conventional affinity measurements for those compounds. By combining information obtained from two different types of fibers, the sensors distinguished between strong and weak estrogens and between estrogenic agonists and an estrogenic antagonist, reflecting strong agreement between sensor results and bioactivity. A patent and three publications are in preparation as a result of this work. Proposed Phase II work will entail: 1) optimization of chemical and molecular biological methods and protocols, 2) refinements in mathematical methods, 3) prototype design and construction, and 4) validation with blind samples of estrogen mimics. The resulting instrument and stabilized reagents provide a rapid assessment of estrogenic and endocrine disrupting potential of samples. This approach may be adapted to pharmaceutical development and identification of non- estrogenic compounds with endocrine-disrupting properties. PROPOSED COMMERCIAL APPLICATION: Agencies monitoring environmental estrogen mimics and screening compounds for estrogenic endocrine disrupting potential, industries monitoring their own waste streams for pseudo-estrogens, pharmaceutical companies seeking anti-breast cancer agents by screening compounds for binding to the estrogen receptor or determining real time binding constants of substances for biomolecules, researchers studying mechanisms of signal transduction.

描述（根据申请人的摘要改编）：回应 提高人们对雌激素模仿作用的意识 生殖障碍和癌症，在第一阶段工作期间 研究人员证明了开发光纤的可行性 筛查雌激素模仿的传感器。 仪器报告快速报告 关于生物大分子及其结合的实时数据 配体。 在几个雌激素和模拟物上进行的传感器测量 与那些传统亲和力测量相关 化合物。 通过结合从两种不同类型获得的信息 在纤维中，传感器区分了强雌激素和弱雌激素 在雌激素激动剂和雌激素拮抗剂之间，反映 传感器结果与生物活性之间的强烈一致。 专利和 由于这项工作，正在准备三个出版物。 拟议的第二阶段工作将需要：1）化学和优化 分子生物学方法和方案，2）改进 数学方法，3）原型设计和构造，以及4） 用雌激素模仿的盲样验证。 结果 仪器和稳定试剂可快速评估 雌激素和内分泌干扰样品的潜力。 这种方法 可以适应非 - 雌激素化合物具有内分泌干扰特性。 拟议的商业应用： 机构监视环境雌激素模仿和筛查 雌激素内分泌破坏潜力的化合物，行业 监视自己的伪雌激素的废物流，药物 通过筛选化合物来寻求反胸腺癌代理商的公司 与雌激素受体结合或确定实时结合 生物分子的物质常数，研究人员 信号转导的机理。